Ena-DATASET-LUMC-CU-30-01-2014-13:09:46:337-6 - samples
Ontology highlight
ABSTRACT:
PROVIDER: EGAD00001000733 | EGA |
REPOSITORIES: EGA
Similar Datasets
Project description:We report on genome-wide DNA methylation differences associated with early gestational famine exposure. We find that open chromatin regions and enhancers are especially sensitive to prenatal famine exposure. We compared 24 individuals conceived during the Dutch Famine, a brief 6 month famine at the end of WWII, with a same sex sibling.
2014-07-01 | E-GEOD-54983 | biostudies-arrayexpress
Project description:This study aims to determine if global gene expression and transcription factor networks in B-lympocytes of siblings with MS were different from healthy siblings. Keywords: Multiple sclerosis, sibling comparisons
2008-05-08 | GSE10064 | GEO
Project description:Transcriptional profiling of hdac1 mutant zebrafish in comparison to their sibling embryos. Embryos resulting from a cross between heterozygous hdac1 mutant zebrafish (hi1618/+) where cultured together then mutants separated from the siblings one the basis of phenotype and RNA extracted from the two groups at 27hpf was compared in a two-colour hybridisation.
2011-01-21 | GSE26707 | GEO
Project description:Transcriptional profiling of hdac1 mutant zebrafish in comparison to their sibling embryos. Embryos resulting from a cross between heterozygous hdac1 mutant zebrafish (hi1618/+) where cultured together then mutants separated from the siblings one the basis of phenotype and RNA extracted from the two groups at 27hpf was compared in a two-colour hybridisation. Two-condition experiment, hdac1 mutants vs. sibling. Biological replicates: 2 (separate mating) Technical replicates: 4 (2 of which are dye-swap)
2011-01-21 | E-GEOD-26707 | biostudies-arrayexpress
Project description:Sensory circuit activation can induce neurotransmitter respecification. To understand the consequences and mechanisms of this neuroplasticity we investigated the effects of olfactory system activation on transmitter expression in interneurons of the accessory olfactory bulb (AOB) during development. Frog larvae use olfactory-mediated kin recognition to distinguish siblings from non-siblings. Prolonged exposure to kin (sibling) or non-kin (non-sibling) odorants changed the number of neurons expressing dopamine or GABA compared to odorant deprivation (orphan condition). To identify signaling molecules mediating this behavior we performed mass spectrometry of kin-conditioned water samples. Vitellogenin-derived peptides, uniquely present in kin-conditioned samples of one genotype, were sufficient to elicit aversion behavior in non-kin larvae. RNA profiling identified AOB microRNAs (miRs) differentially regulated across conditions. Inhibition of miR-375 and miR-200b revealed that they regulate the dopaminergic and GABAergic phenotypes by targeting Pax6 and Bcl11b. Altering the ratio of dopamine/GABA AOB interneurons or locally introducing receptor blockers reversed kinship preference.
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Project description:Microarray transcriptional analysis of 24 individual zebrafish eggs from five sibling mothers
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Project description:The aim of the study is to identify genetic mechanisms of longevity and to characterize their interaction with the environment. Using the results the future health of people without familial longevity can be improved in old age. Long-lived siblings of European descent were recruited together with their offspring and the partners of the offspring. Families were recruited if at least two long-lived siblings were alive and fulfilled the age criterion of 89 years or older for males and 91 years or older for females, representing less than 0.5% of the Dutch population in 2001.
2019-07-11 | MTBLS974 | MetaboLights
Project description:Adults heterozygous for the hi2217 retroviral insertion within the HAI locus (Mathias et al 2007 JCS) were in-crossed for RNA from hi2217 mutants and corresponding WT siblings. Adults heterozygous for the hi1520 retroviral insertion within the Clint1 locus (Dodd et al, 2009) were in-crossed for RNA from hi1520 mutants and corresponding WT siblings. Both hi2217 and hi1520 are mutants with epidermal defects and showing symptoms of chronic inflammation. In this study we aimed to compare the RNA expression changes between Wild-type siblings (a non-inflammatory status) and homozygous mutants (a chronic inflammatory status) for each mutant background (hi2217 and hi1520). Pools of 30-50 zebrafish embryos of WT siblings and hi2217 or hi1520 mutants were collected for RNA isolation. RNA from the hi2217 mutant embryos or hi1520 mutant embryos (test samples)was labelled with Cy5 and hybridized against Cy3-labelled RNA from the corresponding WT sibling embryos (reference samples) . These experiments were performed in biological triplicate.
2014-01-31 | E-GEOD-28110 | biostudies-arrayexpress
Project description:Despite the identification of numerous autism susceptibility genes, the pathobiology of autism remains unknown. The present âcase-controlâ study takes a global approach to understanding the molecular basis of autism spectrum disorders based upon large-scale gene expression profiling. DNA microarray analyses were conducted on lymphoblastoid cell lines from over 20 sib pairs in which one sibling had a diagnosis of autism and the other was not affected in order to identify biochemical and signaling pathways which are differentially regulated in cells from autistic and nonautistic siblings. Bioinformatics and gene ontological analyses of the data implicate genes which are involved in nervous system development, inflammation, and cytoskeletal organization, in addition to genes which may be relevant to gastrointestinal or other physiological symptoms often associated with autism. Moreover, the data further suggests that these processes may be modulated by cholesterol/steroid metabolism, especially at the level of androgenic hormones. Elevation of male hormones, in turn, has been suggested as a possible factor influencing susceptibility to autism, which affects ~4 times as many males as females. Metabolic profiling of steroid hormones in lymphoblastoid cell lines from several pairs of siblings reveals higher levels of testosterone in the autistic sibling, which is consistent with the increased expression of two genes involved in the steroidogenesis pathway. Global gene expression profiling of cultured cells from ASD probands thus serves as a window to underlying metabolic and signaling deficits that may be relevant to the pathobiology of autism. Gene expression profiling of LCL from autistic (21) and nonautistic (17) siblings (4 sets of autistic twins included) were obtained using a custom-printed DNA microarray containing 39,936 elements (TIGR 40K Human array, GPL3427) and a reference design in which each sample was compared to the Stratagene Universal Human RNA standard. Following data normalization, the ratios of expression values for the autistic proband relative to his normal unaffected sibling were determined. Related siblings are identified by their common family ID# (AU****) as provided by the Autism Resource Genetic Exchange (AGRE) repository (and listed in Sample title). Differentially expressed genes were determined across all ratioed expression values for sib pairs (autistic vs. control) using one-class SAM (Statistical Analysis of Microarrays) analysis.
2010-05-15 | E-GEOD-15451 | biostudies-arrayexpress