Dataset Information
The paired FF/FFPE colon set, Exome-Seq - samples
Ontology highlight
ABSTRACT:
PROVIDER: EGAD00001000834 | EGA |
REPOSITORIES: EGA
Publications
Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.
PloS one 20140530 5
Formalin-fixed, paraffin-embedded (FFPE) tissues are an invaluable resource for clinical research. However, nucleic acids extracted from FFPE tissues are fragmented and chemically modified making them challenging to use in molecular studies. We analysed 23 fresh-frozen (FF), 35 FFPE and 38 paired FF/FFPE specimens, representing six different human tissue types (bladder, prostate and colon carcinoma; liver and colon normal tissue; reactive tonsil) in order to examine the potential use of FFPE sam ...[more]
