Ontology highlight
ABSTRACT:
PROVIDER: EGAD00001001386 | EGA |
REPOSITORIES: EGA
Senís Elena E Mockenhaupt Stefan S Rupp Daniel D Bauer Tobias T Paramasivam Nagarajan N Knapp Bettina B Gronych Jan J Grosse Stefanie S Windisch Marc P MP Schmidt Florian F Theis Fabian J FJ Eils Roland R Lichter Peter P Schlesner Matthias M Bartenschlager Ralf R Grimm Dirk D
Nucleic acids research 20160909 1
Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) scaffold, into an engineered genomic miRNA locus. For proof-of-concept, we used TALE or CRISPR/Cas9 nucleases to site-specifically integrate an anti-hepatitis C virus (HCV) shmiRNA into the liver-speci ...[more]