Genomics

Dataset Information

0

Ena-DATASET-Genentech-18-02-2016-19:31:54:532-12 - samples


ABSTRACT: RNA-seq from normal human tissues (2 x 250 bp)

PROVIDER: EGAD00001001922 | EGA |

REPOSITORIES: EGA

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Publications

Prediction and Quantification of Splice Events from RNA-Seq Data.

Goldstein Leonard D LD   Cao Yi Y   Pau Gregoire G   Lawrence Michael M   Wu Thomas D TD   Seshagiri Somasekar S   Gentleman Robert R  

PloS one 20160524 5


Analysis of splice variants from short read RNA-seq data remains a challenging problem. Here we present a novel method for the genome-guided prediction and quantification of splice events from RNA-seq data, which enables the analysis of unannotated and complex splice events. Splice junctions and exons are predicted from reads mapped to a reference genome and are assembled into a genome-wide splice graph. Splice events are identified recursively from the graph and are quantified locally based on  ...[more]

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