Genomics

Dataset Information

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Ena-DATASET-CRUKCI-05-03-2016-10:43:05:168-7 - samples

ABSTRACT: Targeted amplicon sequencing of samples as part of the study "Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples. The amplicon panel consists of 48 amplicons in TP53, PTEN, EGFR, PIK3CA, KRAS and BRAF genes as described previously [Forshew, STM 2012]. All libraries were pooled and quantify using DNA 1000 kit on Agilent 2100 Bioanalyzer and KAPA SYBR FAST ABI Prism qPCR Kit (KAPA Biosystems) on 7900HT Fast Real-Time PCR System (Applied Biosystems) according to the supplier's recommendations. Reads were aligned using bwa-mem v0.7.12-r1039 to the 1000 genomes version of human genome build GRCh37, retaining duplicate reads.

PROVIDER: EGAD00001001937 | EGA |

REPOSITORIES: EGA

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Publications

Development of a Predictive Model for Chewing Difficulty Using EuroQol-5 Dimension Among Korean Older Adults.

Kim Han-Nah HN   Kim Nam-Hee NH  

International journal of dental hygiene 20241104

<h4>Objectives</h4>We aimed to develop a predictive model for chewing difficulty using the EuroQol-5 dimension measure (EQ-5D).<h4>Methods</h4>We included 6643 individuals aged ≥ 65 years (mean age: 72.6 ± 4.96 years; women: 3761 [56.6%]) who had completed the sixth (2013-2015) and seventh (2016-2018) Korea National Health and Nutrition Examination Survey (KNHANES). The participants were further divided into young-old (65-74 years) and old-old (≥ 75 years) adults. Data from the sixth KNHANES wer  ...[more]