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Ena-DATASET-IP-27-09-2016-09:44:13:032-494 - samples

ABSTRACT: We recruited 100 healthy, male donors of self-reported European descent (EUB) and 100 of self-reported African descent (AFB) (Ghent, Belgium). For each participant, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood on Ficoll-Paque density gradients. Monocytes were then positively selected with magnetic CD14 microbeads and exposed for 6 hours to different ligands activating TLR4 (LPS), TLR1/2 (Pam3CSK4), TLR7/8 (R848) and to a human seasonal influenza A virus (IAV). High-quality RNA was obtained from unstimulated and stimulated monocytes for 970 of the 1000 samples (200 x 5 conditions), and was sequenced on an Illumina HiSeq2000. On average, 34 million 101-bp single-end reads were obtained per sample.

PROVIDER: EGAD00001002714 | EGA |

REPOSITORIES: EGA

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Genetic Adaptation and Neandertal Admixture Shaped the Immune System of Human Populations.

Quach Hélène H Rotival Maxime M Pothlichet Julien J Loh Yong-Hwee Eddie YE Dannemann Michael M Zidane Nora N Laval Guillaume G Patin Etienne E Harmant Christine C Lopez Marie M Deschamps Matthieu M Naffakh Nadia N Duffy Darragh D Coen Anja A Leroux-Roels Geert G Clément Frederic F Boland Anne A Deleuze Jean-François JF Kelso Janet J Albert Matthew L ML Quintana-Murci Lluis L

Cell 20161001 3

Humans differ in the outcome that follows exposure to life-threatening pathogens, yet the extent of population differences in immune responses and their genetic and evolutionary determinants remain undefined. Here, we characterized, using RNA sequencing, the transcriptional response of primary monocytes from Africans and Europeans to bacterial and viral stimuli-ligands activating Toll-like receptor pathways (TLR1/2, TLR4, and TLR7/8) and influenza virus-and mapped expression quantitative trait l ...[more]

PMID: 27768888

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