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Ena-DATASET-KNIH-31-07-2017-05:37:25:798-1207 - samples

ABSTRACT: A CKD27_C_Mesan_mRNA-Seq paired end data for Mesangial cells(kidney)

PROVIDER: EGAD00001003484 | EGA |

REPOSITORIES: EGA

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ena-DATASET-KNIH-31-07-2017-05:20:57:234-1194 - samples

Project description:A CKD27_C_Mesan_WGBS paired end data for Mesangial cells(kidney)

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Project description:A CKD23_C_Mesan_mRNA-Seq paired end data for Mesangial cells(kidney)

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Gene expression profiles of the adult mesangial cell (GUDMAP Series_id: 37)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Meis1-GFP BAC transgenic mice were utilized to isolate the mesangial cells from adult kidneys. The mesangial cells were isolated from the glomerulus using collagenase digestion, differential sieving, trypsin treatment and FACS. The RNA was isolated from purified mesangial cells and the gene expression profiles were determined by microarrays.

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