Dataset Information

Ena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:18:36:686-1298 - samples

ABSTRACT: Five hundred fifty nanograms of genomic DNA were input for library preparation after fragmentation by Covaris S2, following the KAPA Hyper Prep Kit (KR0961-V1.14) protocols, with selection for a library size range of 250-450 bp. Three hundred nanograms per library DNA each from 12 samples were normalized and combined into a single pool for exome capture using the xGen Lockdown Probes and Reagents based on their standard protocols

PROVIDER: EGAD00001005754 | EGA |

REPOSITORIES: EGA

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Publications

Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles.

Yan Helen H N HHN Siu Hoi Cheong HC Ho Siu Lun SL Yue Sarah S K SSK Gao Yang Y Tsui Wai Yin WY Chan Dessy D Chan April S AS Wong Jason W H JWH Man Alice H Y AHY Lee Bernard C H BCH Chan Annie S Y ASY Chan Anthony K W AKW Hui Ho Sang HS Cheung Arthur K L AKL Law Wai Lun WL Lo Oswens S H OSH Yuen Siu Tsan ST Clevers Hans H Leung Suet Yi SY

Gut 20200326 12

<h4>Objective</h4>Sporadic early-onset colorectal cancer (EOCRC) has bad prognosis, yet is poorly represented by cell line models. We examine the key mutational and transcriptomic alterations in an organoid biobank enriched in EOCRCs.<h4>Design</h4>We established paired cancer (n=32) and normal organoids (n=18) from 20 patients enriched in microsatellite-stable EOCRC. Exome and transcriptome analysis was performed.<h4>Results</h4>We observed a striking diversity of molecular phenotypes, includin ...[more]

PMID: 32217638

Dataset Information

Ena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:18:36:686-1298 - samples

Publications

Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles.

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