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Ena-DATASET-VUMC-CCA-26-05-2021-09:58:48:372-433 - samples

ABSTRACT: Control cohort of lymphoma samples sequenced with a hybrid capture panel designed to be able to detect translocations and mutations in lymphoma samples. used in the paper "Robust detection of translocations in lymphoma FFPE samples using Targeted Locus Capture-based sequencing"

PROVIDER: EGAD00001007711 | EGA |

REPOSITORIES: EGA

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Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing.

Allahyar Amin A Pieterse Mark M Swennenhuis Joost J Los-de Vries G Tjitske GT Yilmaz Mehmet M Leguit Roos R Meijers Ruud W J RWJ van der Geize Robert R Vermaat Joost J Cleven Arjen A van Wezel Tom T Diepstra Arjan A van Kempen Léon C LC Hijmering Nathalie J NJ Stathi Phylicia P Sharma Milan M Melquiond Adrien S J ASJ de Vree Paula J P PJP Verstegen Marjon J A M MJAM Krijger Peter H L PHL Hajo Karima K Simonis Marieke M Rakszewska Agata A van Min Max M de Jong Daphne D Ylstra Bauke B Feitsma Harma H Splinter Erik E de Laat Wouter W

Nature communications 20210607 1

In routine diagnostic pathology, cancer biopsies are preserved by formalin-fixed, paraffin-embedding (FFPE) procedures for examination of (intra-) cellular morphology. Such procedures inadvertently induce DNA fragmentation, which compromises sequencing-based analyses of chromosomal rearrangements. Yet, rearrangements drive many types of hematolymphoid malignancies and solid tumors, and their manifestation is instructive for diagnosis, prognosis, and treatment. Here, we present FFPE-targeted locu ...[more]

PMID: 34099699

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