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A4ff8fb9-13fb-404d-9cd6-480521f07f5b - samples

ABSTRACT: High-precision human leukocyte antigen (HLA) genotyping is crucial for anti-cancer immunotherapy, but existing tools predicting HLA genotypes using next-generation sequencing (NGS) data are insufficiently accurate. We compared the availability and accuracy of eight HLA genotyping tools (OptiType, HLA-HD, PHLAT, seq2HLA, arcasHLA, HLAscan, HLA*LA, and Kourami) using 1,275 cases from the 1000 Genomes Project data and created a new HLA-genotyping algorithm combining tools. Then, we assessed the new algorithmÃ¢Â€Â™s performance in 39 in-house samples with normal whole-exome sequencing (WES) data and polymerase chain reactionÃ¢Â€Â“sequencing-based typing (PCR-SBT) results.

PROVIDER: EGAD00001007733 | EGA |

REPOSITORIES: EGA

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