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242c1645-5597-49c4-a52f-6e300c9e2a18 - samples

ABSTRACT: Cellular suspensions (Ã¢ÂˆÂ¼15000 cells, with expected recovery of Ã¢ÂˆÂ¼7500 cells) of sorted CD45+ HLA-DR+ CD14+ macrophages from colonic mucosa and muscularis propria were loaded on the 10X Chromium Controller instrument (10X Genomics) according to the manufacturerÃ¢Â€Â™s protocol using the 10X GEMCode proprietary technology. All samples from individual patients were loaded in one batch. The Chromium Single Cell 3Ã‚Â´ v2 Reagent kit (10X Genomics) was used to generate the cDNA and prepare the libraries, according to the manufacturerÃ¢Â€Â™s protocol. The libraries were then equimolarly pooled and sequenced on an Illumina NextSeq500 using HighOutput flow cells v2.5. A coverage of 400M reads per sample was targeted, in order to obtain 50 000 reads per cell. The raw data were then demultiplexed and processed with the Cell Ranger software (10X Genomics) v2.1.1.

PROVIDER: EGAD00001007765 | EGA |

REPOSITORIES: EGA

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