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656d00d5-0e2e-44f3-b6ce-e8787de9b107 - samples

ABSTRACT: To investigate the mode of action and potential side-effects we analyzed differential gene expression in Postmitotic C9orf72 iPSC-Neurons by RNAseq. The cells were treated in a 2 dose regime at 10 Ã‚Âµm in 0.1 % DMSO for 9 days. Compound treated iPSC-Neurons were washed with PBS, frozen on dry ice and stored at -80Ã‚Â°C until RNA isolation. The RNA was isolated using miRNA Mini Kit (Qiagen) using 700 ÃŽÂ¼l of Qiazol. A total of 250 ng of RNA per sample was processed for mRNA library preparation as per the manufacturerÃ¢Â€Â™s instructions for IlluminaÃ‚Â® Stranded mRNA Prep Ligation to be used with the IDTÃ‚Â® for IlluminaÃ‚Â® RNA UD Indexes Set B and sequenced using NextSeq 500/550 High Output Kit v2.5 (Illumina) on NextSeq 550 (Illumina). The data was processed and analyzed using CLC genomics workbench (Version 21.0.3, Qiagen)

PROVIDER: EGAD00001008674 | EGA |

REPOSITORIES: EGA

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