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Db725b02-4c72-4f40-9eea-9ee2a0fa808a - samples

ABSTRACT: The Columbia AlzheimerÃ¢Â€Â™s dataset (white matter) consists of 24 white matter individuals (12 controls, 12 cases). A total of 12,000 estimated cells from each sample were loaded on the 10x Single Cell Next GEM G Chip. cDNA libraries were prepared using the Chromium Single Cell 3Ã¢Â€Â™ Library and Gel Bead v3 kit according to the manufacturerÃ¢Â€Â™s instructions. cDNA libraries were sequenced using the Illumina NovaSeq 6000 System and NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles), aiming at a sequencing depth of minimum 30K reads/nucleus.

PROVIDER: EGAD00001009168 | EGA |

REPOSITORIES: EGA

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