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9e3372d9-7c62-4871-ac4a-67c392e72337 - samples

ABSTRACT: We collected longitudinal samples from 15 patients with MCL at various clinical time points before and after CAT-T therapy brexucabtagene autoleucel (BA) infusion. The patients were grouped into three categories based on their clinical responses after BA treatment: 1) responsive (n =Ã¢Â€Â‰9), 2) relapsed (n =Ã¢Â€Â‰5), and 3) refractory (n =Ã¢Â€Â‰1). All patients in category #1 and #2 had initially attained a complete response (CR) after BA therapy. The responsive group maintained CR with no relapse at the time of last follow up, while the relapsed group achieved initial CR but eventually relapsed. The 10x ChromiumÃ¢Â„Â¢ Single-Cell 5Ã¢Â€Â² Reagent Kit v2 (PN-1000190, 10x GENOMICS) and Chromium Single-Cell Human TCR amplification Kit (PN-1000252, 10x GENOMICS) were used to perform single-cell separation, cDNA amplification, and library construction for gene expression and TCR repertoire following the manufacturerÃ¢Â€Â™s guidelines. Thirty-nine samples passed quality control and underwent single-cell transcriptome profiling with simultaneous single-cell T-cell receptor (TCR) repertoire analysis.

PROVIDER: EGAD00001010180 | EGA |

REPOSITORIES: EGA

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