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Monoclonal_gammopathy - samples

ABSTRACT: Purified plasma cells from bone marrow of Monoclonal gammopathy of unknown significance patient

PROVIDER: EGAD00010000668 | EGA |

REPOSITORIES: EGA

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Whole-epigenome analysis in multiple myeloma reveals DNA hypermethylation of B cell-specific enhancers.

Agirre Xabier X Castellano Giancarlo G Pascual Marien M Heath Simon S Kulis Marta M Segura Victor V Bergmann Anke A Esteve Anna A Merkel Angelika A Raineri Emanuele E Agueda Lidia L Blanc Julie J Richardson David D Clarke Laura L Datta Avik A Russiñol Nuria N Queirós Ana C AC Beekman Renée R Rodríguez-Madoz Juan R JR San José-Enériz Edurne E Fang Fang F Gutiérrez Norma C NC García-Verdugo José M JM Robson Michael I MI Schirmer Eric C EC Guruceaga Elisabeth E Martens Joost H A JH Gut Marta M Calasanz Maria J MJ Flicek Paul P Siebert Reiner R Campo Elías E Miguel Jesús F San JF Melnick Ari A Stunnenberg Hendrik G HG Gut Ivo G IG Prosper Felipe F Martín-Subero José I JI

Genome research 20150202 4

While analyzing the DNA methylome of multiple myeloma (MM), a plasma cell neoplasm, by whole-genome bisulfite sequencing and high-density arrays, we observed a highly heterogeneous pattern globally characterized by regional DNA hypermethylation embedded in extensive hypomethylation. In contrast to the widely reported DNA hypermethylation of promoter-associated CpG islands (CGIs) in cancer, hypermethylated sites in MM, as opposed to normal plasma cells, were located outside CpG islands and were u ...[more]

PMID: 25644835

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Transcription profiling by array of human CD138 purified bome marrow plasma cells from patients with mutliple myeloma or monoclonal gammopathy of unknown significance

Project description:Gene expression profiling of CD138 purified bone marrow plasma cells of previously untreated multiple myeloma patients and individual with monoclonal gammopathy of unknown significance (MGUS)

2010-08-09 | E-MTAB-317 | biostudies-arrayexpress

Transcription profiling by array of human CD138 purified bone marrow plasma cells of previously untreated multiple myeloma patients and individuals with monoclonal gammopathy of unknown significance (MGUS)

Project description:Gene expression profiling of CD138 purified bone marrow plasma cells of previoulsy untreated multiple myeloma patients and individual with monoclonal gammopathy of unknown significance (MGUS)

2010-10-09 | E-MTAB-316 | biostudies-arrayexpress

Gene Expression Profiling in Multiple Myeloma - Reporting of Entities, Risk and Targets in Clinical Routine

Project description:Gene expression profiling of CD138 purified bone marrow plasma cells of previoulsy untreated multiple myeloma patients and individuals with monoclonal gammopathy of uncertain significance (MGUS)

2010-09-08 | E-MTAB-372 | biostudies-arrayexpress

HumanMethylation450K data from Purified Plasma Cells of Monoclonal gammopathy of unknown significance and Multiple myeloma patients and Healthy donors

Project description:HumanMethylation450K data from Purified Plasma Cells of Monoclonal gammopathy of unknown significance and Multiple myeloma patients and Healthy donors

| EGAS00001000841 | EGA

IL21R-expressing CD14+CD16+ monocytes expand in multiple myeloma patients leading to increased osteoclasts

Project description:Bone marrow monocytes are primarily committed to osteoclast formation. It is, however, unknown whether potential primary alterations are specifically present in bone marrow monocytes from patients with multiple myeloma, smoldering myeloma or monoclonal gammopathy of undetermined significance. We analyzed the immunophenotypic and transcriptional profiles of bone marrow CD14+ monocytes in a cohort of patients with different types of monoclonal gammopathies to identify alterations involved in myeloma-enhanced osteoclastogenesis. The number of bone marrow CD14+CD16+ cells was higher in patients with active myeloma than in those with smoldering myeloma or monoclonal gammopathy of undetermined significance. Interestingly, sorted bone marrow CD14+CD16+ cells from myeloma patients were more pro-osteoclastogenic than CD14+CD16-cells in cultures ex vivo Moreover, transcriptional analysis demonstrated that bone marrow CD14+ cells from patients with multiple myeloma (but neither monoclonal gammopathy of undetermined significance nor smoldering myeloma) significantly upregulated genes involved in osteoclast formation, including IL21RIL21R mRNA over-expression by bone marrow CD14+ cells was independent of the presence of interleukin-21. Consistently, interleukin-21 production by T cells as well as levels of interleukin-21 in the bone marrow were not significantly different among monoclonal gammopathies. Thereafter, we showed that IL21R over-expression in CD14+ cells increased osteoclast formation. Consistently, interleukin-21 receptor signaling inhibition by Janex 1 suppressed osteoclast differentiation from bone marrow CD14+ cells of myeloma patients. Our results indicate that bone marrow monocytes from multiple myeloma patients show distinct features compared to those from patients with indolent monoclonal gammopathies, supporting the role of IL21R over-expression by bone marrow CD14+ cells in enhanced osteoclast formation.

2017-04-11 | GSE70345 | GEO

Early alterations in stem-like/marrow-resident T cells, innate and myeloid cells in preneoplastic gammopathy

Project description:We report an alteration in bone marrow immune cell subset composition and function in multiple myeloma and monoclonal gammopathy of unkown significance (MGUS).

2020-12-16 | GSE163278 | GEO

Gene expression analysis of the bone marrow microenvironment reveals distinct immunotypes in smoldering multiple myeloma associated to progression to symptomatic disease

Project description:Gene expression analysis of CD138-depleted bone marrow cells from patients with SMM, patients with monoclonal gammopathy of undetermined significance (MGUS) and patients with symptomatic MM was performed by using Nanostring Technology

2021-12-15 | GSE186537 | GEO

Transcription profiling of human plasma cells from patients with monoclonal gammopathy of undetermined significance, multiple myeloma or plasma-cell leukaemia

Project description:This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 7 monoclonal gammopathy of undetermined significance (MGUS), 39 newly diagnosed multiple myeloma (MM) and 6 plasma-cell leukaemia (PCL) patients. PCs were purified from bone marrow Seriess, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the images were processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed using MAS 5.0 global scaling" procedure, which normalizes the signals of different experiments to the same target intensity (TGT=100).

2007-08-30 | E-GEOD-2113 | biostudies-arrayexpress

Plasma cell dyscrasias expression profiles associated with distinct IGH translocations in multiple myeloma

Project description:This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 7 monoclonal gammopathy of undetermined significance (MGUS), 39 newly diagnosed multiple myeloma (MM) and 6 plasma-cell leukaemia (PCL) patients. PCs were purified from bone marrow Seriess, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the images were processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed using MAS 5.0 "global scaling" procedure, which normalizes the signals of different experiments to the same target intensity (TGT=100). Keywords: other

2005-02-08 | GSE2113 | GEO

DNA methylation in normal PC, MGUS, SMM and MM

Project description:To characterize epigenomic changes during the transformation of normal plasma cells to myeloma, we used the HELP assay to analyze the methylome of CD138+ cells from 56 subjects representing premalignant (MGUS), early and advanced stages of myeloma as well as healthy controls. Plasma cells from premalignant and early stages of myeloma were characterized by striking, widespread hypomethylation. CD138+ selected bone marrow plasma cells from 8 normal donors, 11 patients with monoclonal gammopathy of uncertain significance (MGUS), 4 patients with smoldering myeloma (SMM), 13 patients with newly diagnosed myeloma (NEWMM), 16 patients with relapsed myeloma (REL), including 2 patients with serial samples, and 2 patients in clinical complete remission (REM) were analyzed using the HELP assay [HpaII tiny fragment Enrichment by Ligation-mediated PCR].

2013-04-03 | E-GEOD-43860 | biostudies-arrayexpress

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