Dataset Information

The Finnish National Institute for Health and Welfare

ABSTRACT: The National Institute for Health and Welfare (THL) is a research and development institute under the Finnish Ministry of Social Affairs and Health.THL works to promote the well-being and health of the population, prevent diseases and social problems, and develop social and health services.THL is the statutory statistical authority in health and welfare and maintains a strong knowledge base within its own field of operations.

PROVIDER: EGAO00000000004 | EGA |

REPOSITORIES: EGA

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Publications

An immune response network associated with blood lipid levels.

Inouye Michael M Silander Kaisa K Hamalainen Eija E Salomaa Veikko V Harald Kennet K Jousilahti Pekka P Männistö Satu S Eriksson Johan G JG Saarela Janna J Ripatti Samuli S Perola Markus M van Ommen Gert-Jan B GJ Taskinen Marja-Riitta MR Palotie Aarno A Dermitzakis Emmanouil T ET Peltonen Leena L

PLoS genetics 20100909 9

While recent scans for genetic variation associated with human disease have been immensely successful in uncovering large numbers of loci, far fewer studies have focused on the underlying pathways of disease pathogenesis. Many loci which are associated with disease and complex phenotypes map to non-coding, regulatory regions of the genome, indicating that modulation of gene transcription plays a key role. Thus, this study generated genome-wide profiles of both genetic and transcriptional variati ...[more]

PMID: 20844574

Publication: 1/2

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Project description:Background Bordetella pertussis is a Gram-negative bacterium that infects the human respiratory tract and causes pertussis or whooping cough. The disease has resurged in many countries including Finland where the whole-cell pertussis vaccine has been used for more than 50 years. Antigenic divergence has been observed between vaccine strains and clinical isolates in Finland. To better understand genome evolution in B. pertussis circulating in the immunized population, we developed an oligonucleotide-based microarray for comparative genomic analysis of Finnish strains isolated during the period of 50 years. Methodology/Principal Findings The microarray consisted of 3,582 oligonucleotides (70-mer) and covered 94% of the genome of Tohama I, the strain of which the genome has been sequenced [21]. Twenty isolates from 1953 to 2004 were studied together with two Finnish vaccine strains and two international reference strains. The isolates were selected according to their characteristics, e.g. the year and place of isolation and pulsed-field gel electrophoresis profiles. Genomic DNA of the tested strains, along with reference DNA of Tohama I strain, was labelled and hybridized. The absence of genes as established with microarrays, was confirmed by PCR. Compared to the Tohama I strain, Finnish isolates lost 7 (8.6 kb) to 49 (55.3 kb) genes, clustered in one to four distinct loci. The number of lost genes increased with time, and one third of lost genes had functions related to ion transport, metabolism, or energy production and conversion. All four loci of lost genes were flanked by the insertion sequence element IS481. Conclusion/Significance Our results showed that the progressive gene loss occurred in Finnish B. pertussis strains isolated during a period of 50 years and confirmed that B. pertussis is dynamic and is continuously evolving, suggesting that the bacterium may use gene loss as one strategy to adapt to highly immunized populations. Keywords: comparetive genomic hybridisation

Dataset Information

The Finnish National Institute for Health and Welfare

Publications

An immune response network associated with blood lipid levels.

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