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British Columbia Cancer Agency


ABSTRACT: Organisation EGAO00000000169

PROVIDER: EGAO00000000169 | EGA |

REPOSITORIES: EGA

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Publications

Scalable whole-genome single-cell library preparation without preamplification.

Zahn Hans H   Steif Adi A   Laks Emma E   Eirew Peter P   VanInsberghe Michael M   Shah Sohrab P SP   Aparicio Samuel S   Hansen Carl L CL  

Nature methods 20170109 2


Single-cell genomics is critical for understanding cellular heterogeneity in cancer, but existing library preparation methods are expensive, require sample preamplification and introduce coverage bias. Here we describe direct library preparation (DLP), a robust, scalable, and high-fidelity method that uses nanoliter-volume transposition reactions for single-cell whole-genome library preparation without preamplification. We examined 782 cells from cell lines and triple-negative breast xenograft t  ...[more]

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