CRUKCI
Ontology highlight
ABSTRACT:
PROVIDER: EGAO00000000612 | EGA |
REPOSITORIES: EGA
Publications
Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples.
Piskorz A M AM Ennis D D Macintyre G G Goranova T E TE Eldridge M M Segui-Gracia N N Valganon M M Hoyle A A Orange C C Moore L L Jimenez-Linan M M Millan D D McNeish I A IA Brenton J D JD
Annals of oncology : official journal of the European Society for Medical Oncology 20151217 3
<h4>Background</h4>Next-generation sequencing (NGS) of tumour samples is a critical component of personalised cancer treatment, but it requires high-quality DNA samples. Routine neutral-buffered formalin (NBF) fixation has detrimental effects on nucleic acids, causing low yields, as well as fragmentation and DNA base changes, leading to significant artefacts.<h4>Patients and methods</h4>We have carried out a detailed comparison of DNA quality from matched samples isolated from high-grade serous ...[more]
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