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Mutation tracking in single cell RNA-Seq reveals consequences of subclonal evolution in acute myeloid leukemia

ABSTRACT: Total bone marrow from AML patients was FACS index sorted with staining for Lineage markers (Lin), CD33, CD34, CD38, CD45RA, CD90, CD99, Tim3 and GPR56, and enrichment for Lin- and Lin-CD34+ cells. Single cells were subjected to a modified smart-seq2 protocol with targeting of mutant sites of interest ("MutaSeqÃ¢Â€Â).

PROVIDER: EGAS00001003414 | EGA |

REPOSITORIES: EGA

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