Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Characterization of Hematopoietic Stem and Progenitors Cells in Human Systemic Lupus Eryhtematosus

ABSTRACT: Purpose: Next-generation sequencing (NGS) technology was exploited to map genome-wide expression profile of hematopoietic stem and progenitor cells (HSPCs) in Systemic Lupus Erythematosus (SLE). Results: Transcriptomic analysis of lupus HSPCs indicates activation, displayed increased proliferation and replicative stress, and profoundly prefer to differentiate towards myeloid/granulocytic lineage. Gene expression analysis suggests arrest of differentiation as key regulators including Cebpa, Cebpe, Irf8 are suppressed.

PROVIDER: EGAS00001003679 | EGA |

REPOSITORIES: EGA

ACCESS DATA

Json Xml

Similar Datasets

Next-Generation Sequencing Characterization of Hematopoietic Stem and Progenitors Cells in Murine Systemic Lupus Erythematosus

Project description:Purpose: Next-generation sequencing (NGS) technology was exploited to map genome-wide expression profile of hematopoietic stem and progenitor cells (HSPCs) in mouse model of Systemic Lupus Erythematosus (SLE). Methods: Total RNA was extracted as described by manufacturer (NucleoSpin® RNA XS) and mRNA libraries were generated using the Illumina TruSeq Sample Preparation kit v2. Single-end 75-bp mRNA sequencing was performed on Illumina NextSeq 500 platform. Quality of sequencing was assessed using FastQC software. Raw reads in fastq format were collected and aligned to the mouse genome (mm10 version) using STAR 2.6 algorithm. Gene quantification was performed using HTSeq and differential expression analysis was performed using edgeR package. Results: Transcriptomic analysis of F1-L LSK indicates activation as they respond to type I IFN, displayed increased proliferation and replicative stress, and profoundly prefer to differentiate towards myeloid/granulocytic lineage. Gene expression analysis of F1-L CMPs suggests arrest of differentiation at the level of myeloid progenitors as key regulators including Cebpa, Cebpe, Irf8 are suppressed. Conclusions: Our study suggests deregulation of differentiation at the level of CMPs and “priming” of LSK cells towards granulocytic branch proposing an alternative granulopoiesis route. This comparison indicates an arrest at the progenitor stage both in murine lupus hematopoiesis as important regulators for terminal differentiation are downregulated.

2020-03-17 | GSE128692 | GEO

Systemic lupus erythematosus

Project description:Systemic lupus erythematosus

| PRJNA203032 | ENA

Spatial transcriptomics of Cutaneous and Systemic Lupus Erythematosus skin

Project description:We performed spatial transcriptomics on a case series of different clinical subtypes of cutaneous lupus erythematosus including acute cutaneous lupus erythematosus (malar rash, systemic lupus erythematosus). Our goals were to (1) determine which differentially expressed genes (DEGs) could be attributed to specific cell populations in specific locations within the tissue, (2) determine if spatial transcriptomics could better distinguish between CLE clinical subtypes than bulk RNA approaches and (3) examine potential cell-cell communication pathways within the skin lesions.

2021-10-01 | GSE182825 | GEO

Systemic Lupus Erythematosus patient blood with controls

Project description:The experiment consists of 31 Systemic Lupus Erythematosus patient blood samples and 29 healthy donor blood samples.

2018-03-21 | GSE112087 | GEO

SWATH-MS based protein quantification for diagnosis in systemic lupus erythematous

Project description:The gold standard for diagnosing lupus nephritis, one of the common symptoms of systemic lupus erythematosus, is an invasive procedure, a Renal biopsy. To overcome the risk of invasive methods, we constructed a spectral library using biological samples that can be obtained non-invasively and analyzed it using the SWATH-MS method, a quantitative proteomic analysis.

2023-10-24 | PXD040099 | Pride

DNA methylation in systemic lupus erythematosus patients

Project description:To screen specific DNA methylation markers in systemic lupus erythematosus (SLE) patient's blood DNA, whole-blood DNAs from 6 female SLE patients and 6 female controls were analyzed by methylation microarray.

2016-12-27 | GSE57869 | GEO

Human peripheral blood cells: systemic lupus erythematosus vs healthy individual.

Project description:Gene expression profiling of peripheral blood cells from patients with systemic lupus erythematosus (SLE) vs healthy individual (HI).

2010-10-01 | GSE20864 | GEO

High-Density Lipoproteins - small RNA Signatures in Systemic Erythematosus Lupus.

Project description:Study of high-density lipoproteins using 6 human plasma samples. The study sought to find small RNA signatures in systemic erythematosus lupus.

2018-10-27 | GSE121865 | GEO

Human peripheral blood cells: systemic lupus erythematosus vs healthy individual.

Project description:Gene expression profiling of peripheral blood cells from patients with systemic lupus erythematosus (SLE) vs healthy individual (HI). Peripheral blood was obtained from patients with SLE (n=21) and HI (n=45). Blood samples from 45 HI are used as control.

2010-10-01 | E-GEOD-20864 | biostudies-arrayexpress

Gut microbiota in systemic lupus erythematosus

Project description:Gut microbiota in systemic lupus erythematosus

| PRJEB52971 | ENA