Imaging metabolic heterogeneity in breast cancer using hyperpolarized 13C-MRI
Ontology highlight
ABSTRACT:
PROVIDER: EGAS00001004118 | EGA |
REPOSITORIES: EGA
Similar Datasets
Project description:Stress constantly challenges plant adaptation to the environment. Of all stress types, arsenic was a major threat during the early evolution of plants. The most prevalent chemical form of arsenic is arsenate, whose similarity to phosphate renders it easily incorporated into cells via the phosphate transporters. Here we found that arsenate stress provokes a notable transposon burst in plants, in coordination with arsenate/phosphate transporter repression, which immediately restricts arsenate uptake. This repression was accompanied by delocalization of the phosphate transporter from the plasma membrane. When arsenate was removed, the system rapidly restored transcriptional expression and membrane localization of the transporter. We identify WRKY6 as an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation. Plants therefore have a dual WRKY-dependent signaling mechanism that modulates arsenate uptake and transposon expression, providing a coordinated strategy for arsenate tolerance and transposon gene silencing. Three biological replicates were performed for each sample type. Single channel hybridizations were carried-out using either Affymetrix ATH1 platform or Nimblegen Gene Expression 12x135K platform (Arabidopsis thaliana).
2014-04-25 | E-GEOD-49037 | biostudies-arrayexpress
Project description:Microarray analyses revealed strong correlations between firmness of fillet (most important quality parameter) and a large number of genes. Highly similar expression profiles were observed in several functional groups. Positive regression was found between firmness and genes encoding proteasome components (41 genes) and mitochondrial proteins (129 genes), proteins involved in stress responses (12 genes) and lipid metabolism (30 genes). A weaker though highly significant negative regression was seen in sugar metabolism (26 genes) and myofiber proteins (42 genes). Among individual genes that showed a strong association with firmness, there were extracellular matrix proteins (negative correlation), immune genes and intracellular proteases (positive correlation). Several genes can be regarded as candidate markers of flesh quality (coiled-coil transcriptional coactivator b, AMP deaminase 3 and oligopeptide transporter 15) though their functional roles are unclear. Fillet firmness in a population of farmed Atlantic salmon with known pedigree. Sixteen individuals were chosen to cover the normal range of this trait.
2012-03-14 | E-GEOD-36475 | biostudies-arrayexpress
Project description:Microarray analyses revealed strong correlations between firmness of fillet (most important quality parameter) and a large number of genes. Highly similar expression profiles were observed in several functional groups. Positive regression was found between firmness and genes encoding proteasome components (41 genes) and mitochondrial proteins (129 genes), proteins involved in stress responses (12 genes) and lipid metabolism (30 genes). A weaker though highly significant negative regression was seen in sugar metabolism (26 genes) and myofiber proteins (42 genes). Among individual genes that showed a strong association with firmness, there were extracellular matrix proteins (negative correlation), immune genes and intracellular proteases (positive correlation). Several genes can be regarded as candidate markers of flesh quality (coiled-coil transcriptional coactivator b, AMP deaminase 3 and oligopeptide transporter 15) though their functional roles are unclear.
2012-03-14 | GSE36475 | GEO
Project description:Stress constantly challenges plant adaptation to the environment. Of all stress types, arsenic was a major threat during the early evolution of plants. The most prevalent chemical form of arsenic is arsenate, whose similarity to phosphate renders it easily incorporated into cells via the phosphate transporters. Here we found that arsenate stress provokes a notable transposon burst in plants, in coordination with arsenate/phosphate transporter repression, which immediately restricts arsenate uptake. This repression was accompanied by delocalization of the phosphate transporter from the plasma membrane. When arsenate was removed, the system rapidly restored transcriptional expression and membrane localization of the transporter. We identify WRKY6 as an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation. Plants therefore have a dual WRKY-dependent signaling mechanism that modulates arsenate uptake and transposon expression, providing a coordinated strategy for arsenate tolerance and transposon gene silencing.
2014-04-25 | GSE49037 | GEO
Project description:Triangular Correlation (TrC) between cancer aggressiveness, cell uptake capability and cell deformability
| PRJNA557700 | ENA
Project description:Dietary fat quality may influence skeletal muscle lipid handling and fat accumulation, thereby modulating insulin sensitivity. Objective: To examine acute effects of meals with various fatty acid (FA) compositions on skeletal muscle FA handling and postprandial insulin sensitivity in obese insulin resistant men. Design: In a single-blinded randomized crossover study, 10 insulin resistant men consumed three high-fat mixed-meals (2.6MJ). Meals were high in saturated FA (SFA), in monounsaturated FA (MUFA) or in polyunsaturated FA (PUFA). Fasting and postprandial skeletal muscle FA handling were examined by measuring arterio-venous concentration differences across forearm muscle. [2H2]-palmitate was infused intravenously to label endogenous triacylglycerol (TAG) and FFA in the circulation and [U-13C]-palmitate was added to the meal to label chylomicron-TAG. Skeletal muscle biopsies were taken to assess intramuscular lipid metabolism and gene expression. Results: Insulin and glucose responses (AUC) after SFA meal were significantly higher compared with PUFA meal (p=0.003 and 0.028, respectively). Uptake of TAG-derived FA was significantly lower in the early postprandial phase after PUFA meal as compared with other meals (AUC60-120, p<0.001). The PUFA meal induced less transcriptional downregulation of oxidative pathways compared with other meals. The fractional synthetic rate was higher in DAG and PL fraction after MUFA and PUFA meal. Conclusion: Intake of a PUFA meal reduced TAG-derived skeletal muscle FA uptake, which was accompanied by higher postprandial insulin sensitivity and a tendency towards a higher muscle lipid turnover. These data suggest that the effects of replacement of SFA by PUFA may contribute to less muscle lipid uptake and may be therefore protective against the development of insulin resistance. Keywords: expression profiling by array randomized crossover dietary intervention study
2012-02-21 | E-GEOD-31901 | biostudies-arrayexpress
Project description:As a sextual behavior, competence or natural transformation mediates lateral gene transfer, and thereby promotes exchange of antibiotic resistance and virulence traits among bacteria. Competence is assoicated with activation of many genes, rapid build-up of membrane-bound DNA uptake apparatus, and transient growth arrest. It is completely unknown how transformable bacteria recover from the competence-associated status. This work reveals that the membrane-associated HtrA protease enables the pneumococcus (an important human pathogen) to recover from the competence-associated status by breaking down the DNA uptake apparatus. This is achieved by selective degradation of ComEA and ComEC, the major components of the DNA uptake apparatus. Deleting htrA and/or over-expressing comEA-EC led to dramatic delay in competence termination. Based on high conservation of HtrA and DNA uptake proteins among transformable bacterial, the HtrA protease may serve as a general regulator of bacteria recovery from the competence-associated status.
2018-03-22 | GSE112169 | GEO