Project description:Single-cell RNA sequencing was performed on embryonic Drosophila heart cells. Analysis of single-cell RNA sequence (scRNA-seq) data at timepoints prior to migration of cardiac progenitor cells through to heart tube closure (embryonic stages 13, 14-early, 14-late, 15 and 16) revealed several interesting findings. We found specification of cardiac cell types takes place early, before stage 13, with biggest changes in transcriptomic profiles detected once cells had settled at the midline for further cardioblast maturation. Throughout development, our data identified multiple cell types, covering cardioblasts and five types of pericardial cells including a neural cardiac cell type. The scRNA-seq data further revealed a combination of first and second heart fields during heart development in fly. Further, we uncovered new cell type-specific markers discerning the different cardiac cell types. And we identified signaling pathways key to heart cell maturation, which are conserved from fly to human.
Project description:Development of specialized cell types and structures in the vertebrate heart is regulated by spatially-restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development and function we used tomo-seq, combining high-throughput RNA sequencing with tissue sectioning, to establish a genome-wide expression dataset with high spatial resolution for the developing zebrafish heart. Analysis of the dataset revealed over 1100 genes differentially expressed in sub-compartments. Pacemaker cells in the sinoatrial region induce heart contractions, but little is known about the mechanisms underlying their development and function. Using our transcriptome map, we identified spatially restricted Wnt/β-catenin signaling activity in pacemaker cells, which was controlled by Islet-1 activity. Moreover, Wnt/β-catenin signaling at a specific developmental stage in the myocardium controls heart rate by regulating pacemaker cellular response to parasympathetic stimuli. Thus, this high-resolution transcriptome map incorporating all cell types in the embryonic heart can expose spatially-restricted molecular pathways critical for specific cardiac functions.
Project description:We report bulk RNA-sequencing, ChIP-seq, and ATAC-seq of endothelial cells harvested from heart and lung of multiple mouse strains investigating the role of KLF2 and KLF4 in endothelial transcription
Project description:Heart biopsies of explanted organ after heart failure. Same patient, 3 different sample types. 1,2,6 - from paraffin tissue, Thermo fisher kit 3,4,5 - from paraffin tissue, Qiagen kit 7,8,9 - fresh tissue with Qiagen kit
Project description:The vertebrate heart is the first organ to form in the embryo and is composed of mesodermal progenitors that arise in an area termed the cardiac crescent. These give rise not only to muscle cells but also to a variety of other cell types, all of which work together to allow the heart to beat rhythmically. Current understanding of when and how these different cell types arise during early cardiogenesis is limited. Therefore, we microdissected the cardiac crescent region of mouse embryos at different stages of development -from when the structure is first present until the linear heart tube (LHT) stage- and performed single-cell RNA-sequencing. The present submission contains pilot data from the LHT.