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Transcriptomic analysis of TFEB overexpression in LT-HSC, ST-HSC and MEP

ABSTRACT: Long-term hematopoietic stem cells (LT-HSC), Short-term hematopietic stem cells (ST-HSC) and Megakaryocyte-erythrocyte progenitors (MEP) were sorted from human cord blood and cultured overnight before transduction with lentivirus to overexpress GP91 (CTRL) or TFEB. Three (LT-HSC ans ST-HSC) or six (MEP) days later, BFP+ transduced cells were sorted for RNA extraction and sequencing.

PROVIDER: EGAS00001004969 | EGA |

REPOSITORIES: EGA

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