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Investigation of different library preparation and tissue of origin deconvolution methods for urine and plasma cfDNA methylome analysis

ABSTRACT: In this study, a single- and a double-stranded library preparation approach was evaluated with respect to their technical biases when applied on cfDNA from plasma and urine. This study thus outlines potential limitations of double-stranded library preparation for methylation analysis of cfDNA especially for urinary cfDNA. While the double-stranded method allows jagged end analysis in addition to TOO analysis, it leads to significant methylation bias in urinary cfDNA, which single-stranded methods can overcome.

PROVIDER: EGAS00001007314 | EGA |

REPOSITORIES: EGA

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