Transcriptomics

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Human TFIIH kinase CDK7 regulates transcription-associated epigenetic modification


ABSTRACT: CDK7 phosphorylates the RNA polymerase II (pol II) CTD and activates the P-TEFb- associated kinase, CDK9, but its regulatory roles remain obscure. Using human CDK7 analog-sensitive (CDK7as) cells, we observed reduced capping enzyme recruitment, increased pol II promoter-proximal pausing, and defective termination at gene 3'-ends upon CDK7 inhibition. We also found that CDK7 regulates chromatin modifications downstream of transcription start sites. H3K4me3 spreading was restricted at gene 5'-ends and H3K36me3 was displaced toward gene 3'-ends in CDK7as cells. Together, these results implicate a CDK7-dependent "CTD code" that regulates epigenetic marks in addition to RNA processing and pol II pausing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE100040 | GEO | 2017/08/01

SECONDARY ACCESSION(S): PRJNA390543

REPOSITORIES: GEO

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