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Cross-linking and immunoprecipitation (CLIP) analysis for identifying binding sites of Tet2 in bone marrow derived mast cell


ABSTRACT: To transcriptome-widely identify the mRNA targets of Tet2 and investigate the immunological function of Tet2 at RNA regulation level in vivo, we performed CLIP-seq of Tet2 in BMMCs. We performed three bio-replicates of CLIP-seq for getting potential Tet2-binding mRNAs.Using data of normalized tag numbers in the common peaks, we found that biological replicates correlated well with each other.More than 60% of the peaks located in genic regions, which were preferentially enriched in coding sequencing (CDS) among mature mRNA elements.

ORGANISM(S): Mus musculus

PROVIDER: GSE100559 | GEO | 2018/01/25

REPOSITORIES: GEO

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