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Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop2

ABSTRACT: The hypothesis of the experiment is that infected trees of high resistance express a wider variety of resistance genes than infected trees of low resistance, and that the level of expression of these resistance genes differs between infected and healthy branches. Also, some genes highly expressed in the infected state not expressed in the healthy state may be in response to the wounding rather than the actual infection. By comparing these expressions to that of wounded, uninfected branches, this could also be clarified. Keywords: stress response

ORGANISM(S): Picea abies Pinus taeda

PROVIDER: GSE10059 | GEO | 2008/05/03

SECONDARY ACCESSION(S): PRJNA108959

REPOSITORIES: GEO

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Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop1

2008-05-03 | GSE10058 | GEO

Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection (Loops 1 and 2)

2008-05-03 | GSE11323 | GEO

Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop1

Project description:The hypothesis of the experiment is that infected trees of high resistance express a wider variety of resistance genes than infected trees of low resistance, and that the level of expression of these resistance genes differs between infected and healthy branches. Also, some genes highly expressed in the infected state not expressed in the healthy state may be in response to the wounding rather than the actual infection. By comparing these expressions to that of wounded, uninfected branches, this could also be clarified. Three different Picea abies clones, of increasing resistance to fungal attack, have been infected. For all clones, three rametes have been infected with Heterobasidion of 1 month, by means of cutting the cambium and inoculating a piece of fungus growing in agar in the wound. For the low and medium resistant clone, a fourth ramete has been cut and inoculated with agar not containing any fungal material.

2008-05-03 | E-GEOD-10058 | biostudies-arrayexpress

Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop2

2008-05-03 | E-GEOD-10059 | biostudies-arrayexpress

Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection (Loops 1 and 2)

Project description:This SuperSeries is composed of the following subset Series: GSE10058: Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop1 GSE10059: Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop2 The hypothesis of the experiment is that infected trees of high resistance express a wider variety of resistance genes than infected trees of low resistance, and that the level of expression of these resistance genes differs between infected and healthy branches. Also, some genes highly expressed in the infected state not expressed in the healthy state may be in response to the wounding rather than the actual infection. By comparing these expressions to that of wounded, uninfected branches, this could also be clarified. Refer to individual Series

2008-05-03 | E-GEOD-11323 | biostudies-arrayexpress

Heterotrimeric G-proteins in Picea abies and their regulation in response to Heterobasidion annosum s.l. infection.

Project description:Heterotrimeric G-proteins are important signalling switches, present in all eukaryotic kingdoms. In plants they regulate several developmental functions and play an important role in plant-microbe interactions. The current knowledge on plant G-proteins is mostly based on model angiosperms and little is known about the G-protein repertoire and function in other lineages. In this study we investigate the heterotrimeric G-protein subunit repertoire in Pinaceae, including phylogenetic relationships, radiation and sequence diversity levels in relation to other plant linages. We also investigate functional diversification of the G-protein complex in Picea abies by analysing transcriptional regulation of the G-protein subunits in different tissues and in response to pathogen infection.A full repertoire of G-protein subunits in several conifer species were identified in silico. The full-length P. abies coding regions of one G?-, one G?- and four G?-subunits were cloned and sequenced. The phylogenetic analysis of the G?-subunits showed that PaGG1 clustered with A-type-like subunits, PaGG3 and PaGG4 clustered with C-type-like subunits, while PaGG2 and its orthologs represented a novel conifer-specific putative G?-subunit type. Gene expression analyses by quantitative PCR of P. abies G-protein subunits showed specific up-regulation of the G?-subunit gene PaGPA1 and the G?-subunit gene PaGG1 in response to Heterobasidion annosum sensu lato infection.Conifers possess a full repertoire of G-protein subunits. The differential regulation of PaGPA1 and PaGG1 indicates that the heterotrimeric G-protein complex represents a critical linchpin in Heterobasidion annosum s.l. perception and downstream signaling in P. abies.

| S-EPMC4676809 | biostudies-literature

Transcript profiling of Pinus sylvestris trees as a response to Heterobasidion annosum infection under field conditions

Project description:In conifer forests of Northern Europe, a pathogenic fungus Heterobasidion annosum attacks the roots of Scots pine and causes mortality. Trees with infection grow slower and produce less timber with reduced quality. Despite applied control methods, such as switching tree species to a non-host species, or stump treatment, root and butt rot continues to be a serious forest health problem. Disease resistance breeding is a less-applied control method which has potential to improve tree health. However, neither conifer genotypes with absolute resistance to Heterobasidion sp. nor robust selection markers for resistance breeding have been found. We studied the responses of various Scots pine genotypes to Heterobasidion annosum infection and mechanic damage in drained peatland. Stems and roots of mature naturally regenerated Scots pine trees growing in drained peatland were either artificially infected with H. annosum or wounded and inoculated with sterile inoculum. Untreated trees from the study sites served as controls. Responses of different Scots pine genotypes to pathogen infection as determined by lesion size were recorded from samples harvested four months after inoculation, and least susceptible and highly susceptible genotypes were selected from the study material. Analysis of terpenoids from both least susceptible and highly susceptible pine genotypes by gas chromatography coupled with mass spectrometry indicates that some monoterpenes and sesquiterpenes are differentially induced depending on the susceptibility level. Transcriptomic microarray analysis was therefore conducted with RNA from stems of the least susceptible and highly susceptible Scots pine genotypes. Gene expression data from cDNA microarray were analysed by comparisons between the treatments, and the genotypes with different resistance level. The aim of the study is to highlight transcripts specific to differing levels of susceptibility.

2019-02-01 | GSE66168 | GEO

Transcriptional response of Scots pine trees to Heterobasidion annosum infection: analyses of antimicrobial protein Sp-AMP2

Project description:Understanding of mechanisms of resistance of forest trees against microbial pathogens is an essential prerequisite for the development of sustainable forestry practices and for the improvement of commercially-grown trees via either conventional breeding or rational genetic engineering. We have studied the transcriptional response of Scots pine trees to Heterobasidion annosum infection under field conditions. By comparing responses of trees to wounding and to fungal inoculation we could identify a set of genes that were specifically responding to fungal infection. We have also investigated a contribution of Scots pine antimicrobial protein Sp-AMP2 to the host antimicrobial defense to evaluate the potential of Sp-AMP genes as molecular markers for resistance breeding.

2017-12-31 | GSE87881 | GEO