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HMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export VI

ABSTRACT: To examine whether the competition between hMTR4 with ALYREF is important for the specific exosome recruitment, we performed stranded RNA-seq using rRNA-depleted nuclear RNAs isolated from ALYREF and control overexpression cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE100982 | GEO | 2017/12/06

SECONDARY ACCESSION(S): PRJNA393570

REPOSITORIES: GEO

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export V

Project description:To examine whether the competition between hMTR4 with AlyREF is important for the specific exosome recruitment,we sequenced the RNAs associating with hMTR4 in control and AlyREF overexpression cells.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export III

Project description:To eliminate the possibility that for a particular gene the increased RNA level is an effect of enhanced transcription. We carried out ChIP-seq for RNAPII to compare transcription of mRNAs and lncRNAs in control, hRRP40 and hMTR4 knockdown cells. Among 15000 RNAPII binding peaks identified in these samples, only less than 50 peaks was significantly increased in hRRP40 or hMTR4 knockdown cells.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export

Project description:This SuperSeries is composed of the SubSeries listed below.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export IV

Project description:To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using rRNA-depleted RNAs isolated from HeLa cells.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export II

Project description:To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using polyA RNAs isolated from nuclei of HeLa cells. To compare the RNA levels in each sample in an unbiased fashion, we added spike-in controls to equal amount of total nuclear RNAs.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export I

Project description:To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using rRNA-depleted RNAs isolated from nuclei of HeLa cells. To compare the RNA levels in each sample in an unbiased fashion, we added spike-in controls to equal amount of total nuclear RNAs.

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hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export

Project description:hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export

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NRDE2 negatively regulates nuclear exosome functions II

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect nuclear RNAs isolated from NRDE2 and control knockdown cells.

2019-03-14 | GSE120203 | GEO

NRDE2 negatively regulates nuclear exosome functions I

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect total RNAs isolated from NRDE2 and control knockdown cells.

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NRDE2 negatively regulates nuclear exosome functions IV

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect RNAs isolated from MTR4 IP in NRDE2 and control knockdown cells.

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