Transcriptome profiling of the testis developing and spermatogenesis of Mongolian horse using RNA-Seq
Ontology highlight
ABSTRACT:
ORGANISM(S): Equus caballus
PROVIDER: GSE101697 | GEO | 2017/07/21
SECONDARY ACCESSION(S): PRJNA395221
REPOSITORIES: GEO
Similar Datasets
Project description:PIWI-interacting RNAs (piRNAs), have been identifed in mammalian testes, and were essential for development of testis and spermatogenesis.
2017-07-07 | GSE100852 | GEO
Project description:BackgroundHorse testis development and spermatogenesis are complex physiological processes.MethodsTo study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis.ResultsA total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to "spermatogenesis," male gamete generation," "spermatid development" and "oocyte meiosis" were significantly involved in different stages of testis development and spermatogenesis.ConclusionGenes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
| S-EPMC7187496 | biostudies-literature
Project description:Vertebrate spermatogonial stem cells maintain sperm production over the lifetime of an animal but fertility declines with age. While morphological studies have informed our understanding of typical spermatogenesis, the molecular and cellular mechanisms underlying the maintenance and decline of spermatogenesis are not yet understood. We used single-cell RNA sequencing to generate a developmental atlas of the aging zebrafish testis. All testes contained spermatogonia, but we observed a progressive decline in spermatogenesis that correlates with age. Testes from some older males only contained spermatogonia and a reduced population of spermatocytes. Spermatogonia in older males are transcriptionally distinct from spermatogonia in testes capable of robust spermatogenesis. Immune cells including macrophages and lymphocytes drastically increase in abundance in testes that cannot complete spermatogenesis. Our developmental atlas reveals the cellular changes as the testis ages and defines a molecular roadmap for the regulation of spermatogenesis.
2024-10-04 | GSE275361 | GEO
Project description:Our experimental design includes samples at different time points during the first wave of spermatogenesis. Each time point corresponds to specific cell content in the testis. First time point is post natal day (PND) 7, when only somatic cells and spermatogonia are present in the testis. Thereafter additional cell types are present in selected samples in chronological order: PND 14 contains early spermatocytes, PND 17 late spermatocytes, PND 21 round spermatids and finally PND 28 elongating spermatids. Our results highlight differentially expressed genes and gene isoforms, which are important for correct sperm development and male fertility. In addition, functional analysis suggests a highly controlled gene expression pattern during the first wave of spermatogenesis. Transcriptome sequencing of the mouse testes at PND 7, 14, 17, 21 and 28 for analysis of differences in gene expression pattern during the first wave of spermatogenesis. In total 10 samples were analysed, replicates for each time point.
2013-04-29 | E-GEOD-39970 | biostudies-arrayexpress
Project description:The Mongolian horse has the characteristics of cold resistance, rough feeding resistance, good endurance, strong disease resistance and strong gregariousness. The normal production of sperm in male mammals is the basis of reproduction, and spermatogenesis includes a mitosis, meiosis twice and metamorphosis of sperm cells. The MEI1 gene is involved in the meiosis cycle and is required for normal meiosis chromosome association. Previous studies have shown that alternative splicing of MEI1 gene can promote spermatogenesis in Mongolian horses. In this study, the expression of MEI1 gene was determined by immunofluorescence in the third generation Mongolian horse testicular sertoli cells. Two overexpressed lentiviral vectors with MXE and SE events of MEI1 gene were constructed and successfully infected into sertoli cells. CCK-8 was used to detect the proliferation and activity of infected cells, transcripomics and metabolomics were used to screen and annotate differential genes and differential metabolites in the two sertoli cells with MXE and SE events. After 72 h of virus infection, 193 differentially expressed genes and 11360 differentially expressed metabolites were screened, 109 up-regulated genes including MEI1 and 84 down-regulated genes, 7494 up-regulated metabolites and 3866 down-regulated metabolites. Differential genes and differential metabolites were mainly enriched in several pathways related to spermatogenesis. Differential genes such as IL31RA, ATP2B3, CASQ2 were highly expressed in SE events, IL11, PRLR, CCR7 were highly expressed in MXE events. Metabolites such as folic acid and spermine were highly expressed in SE events, citric acid and glutathione were highly expressed in MXE events. This suggested that both MXE and SE events of MEI1 gene can promote the activity of spermatogenesis signaling pathway, and the gene influences spermatogenesis by regulating the expression of metabolites. In summary, MXE and SE events of MEI1 gene can promote the expression of spermatogen-related genes and metabolites, and genes affect spermatogenesis by regulating the expression of metabolites. The results of this study laid a theoretical foundation for exploring the regulatory mechanism of different alternative splicing events on spermatogenesis in Mongolian horse.
2024-02-02 | GSE254391 | GEO
Project description:Cultivation of neonatal mouse testis tissue can induce spermatogenesis and produce fertile sperms. However, this in vitro spermatogenesis by the current organ culture method comes short in fully mimicking the in vivo counterpart, partly due to a lack of knowledge about the underlying molecular phenotypes. In this study, we investigated transcriptome of cultured testis tissues using microarray method. Principle component analysis of the transcriptome data revealed delay and/or arrest of spermatogenesis and immediate radical immune reactions in the cultured testis tissues. The delay/arrest of spermatogenesis occurred before and during early meiotic phase, resulting in inefficient progression of meiosis. The immune reaction, on the other hand, was drastic and overwhelming, in which TLR4-NF-kB signaling was speculated to be involved. Notably, treatment with TAK242, an inhibitor of TLR4-NF-kB signaling pathway, ameliorated the macrophage activation which otherwise would exacerbate the inflammation. The present study revealed for the first time the immense immune reaction in the cultured tissue by transcriptomic analysis, which could be a cause of the inefficiency of in vitro spermatogenesis.
2020-06-30 | GSE147982 | GEO
Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
2021-12-01 | GSE142725 | GEO
Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
2021-12-01 | GSE142724 | GEO
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.
2021-12-01 | GSE142795 | GEO
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.
2021-12-01 | GSE142793 | GEO