Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genomic occupancy profile of Rbf and Dp in flight muscles of WT Drosophila pharate

ABSTRACT: Our study focuses on understanding the genomic occupancy profile of Rbf and Dp in flight muscles of D. melanogaster. We analyzed Rbf and Dp occupancy by ChIP-seq using Rbf and Dp antibodies in dissected flight muscles of pharate flies (WT)

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE102043 | GEO | 2019/01/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional profile of larval notum and flight muscle in Rbf knockdown and WT Drosophila flies

Project description:Our study focuses on understanding how Rbf impacts the formation of flight muscles in Drosophila. We analyzed by RNA-seq the transcriptional changes dependent on Rbf in proliferating adult muscle precursors and in mature flight muscles at two different stages of Drosophila development

2019-01-04 | GSE102104 | GEO

Drosophila melanogaster

Project description:Genomic occupancy profile of Rbf and Dp in flight muscles of WT Drosophila pharate

| PRJNA396483 | ENA

Systematic transcriptomics reveals a biphasic mode of sarcomere morphogenesis in flight muscles regulated by Spalt

Project description:Muscles organise a pseudo-crystalline array of actin, myosin and titin filaments to build force-producing sarcomeres. To study how sarcomeres are built, we performed mRNA-sequencing of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, two clusters are strongly enriched for sarcomeric components. Temporal gene expression together with detailed morphological analysis enabled us to define two distinct phases of sarcomere development, both of which require the transcriptional regulator Spalt major. During the first sarcomere formation phase, 2.0 µm long immature sarcomeres assemble myofibrils that spontaneously contract. In the second sarcomere maturation phase, sarcomeres grow to their final 3.2 µm length and 1.5 µm diameter and acquire stretch-sensitivity. Interestingly, the final number of myofibrils per flight muscle fiber is determined at the onset of the first phase and remains constant. Together, this defines a biphasic mode of sarcomere and myofibril morphogenesis – a new concept which may also apply to vertebrate muscle or heart development.

2017-12-01 | GSE107247 | GEO

E2F regulation of Phosphoglycerate kinase (Pgk) gene is functionally important in Drosophila development

Project description:Our study focuses on understanding the changes in chromatin accessibility in the Pgk-DeltaE2F mutant that takes place during flight muscles development in Drosophila. E2F binding sites were mutated upstream Pgk gene. E2F/Dp/Rbf were no longer recruited to this regulatory region. As a result of this, the expression of Pgk mRNA was downregulated, which led to reduced levels in many metabolites that are glycolytic and TCA intermediates. Using ATAC-seq we determined that there was a global reduction in chromatin accessibility in the mutant compared to control, and it was accompagnied by a widespread reduction in gene expression, including metabolic genes. In addition we identified a broad range of phenotypes when analyzing the functional relevance of E2F regulation on Pgk gene. These phenotypes range from low ATP content, abnormal mitochondrial morphology, shortened life span to embrionic lethality. Furthermore, defects in high-energy consuming organs, such as ovaries and muscles, were found. In sum, our results illustrate the pleiotropic effects on metabolism, gene expression and development in the Pgk-deltaE2F animals, which underlies the importance of E2F regulation on a single E2F target, Pgk.

2023-03-20 | GSE217225 | GEO

The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle

Project description:In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between cytoplasm and nuclei, and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific transcription and splicing of various sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may regulate mammalian muscle morphogenesis. 9 samples from Drosophila melanogaster were analyzed in duplicate: control dissected wildtype flight muscle at 30h APF, 72h APF and 0 day adult, jump muscle and whole leg from 1d adult and RNAi/mutant conditions for salm (1d flight muscle) and aret (30h, 72h and 1d flight muscle)

2014-12-23 | E-GEOD-63707 | biostudies-arrayexpress

The impact of E2F/Dp inactivation on metabolism differs between muscles and fat body cells

Project description:We aim to determine the physiological impact of inactivating E2F in muscles and in adipose cells in vivo. To uncover the mechanism of action of E2F in each tissue we investigated the similarities between E2F-deficient tissues by comparing the proteomic profiles between muscles and fat cells. Our findings revealed that E2F is required in fat cells to promote the storage of fat and the synthesis of trehalose, which was restored by feeding animals in high sugar diet. Multiplexed quantitative proteomics was performed using TMT10-plex reagents on an Orbitrap Fusion mass spectrometer using the SPS-MS3 method. Two sample sets - fatbody samples and samples of thoracic muscles - were analyzed using two TMT sets. Twelve high pH reversed-phase chromatography fractions were analyzed per sample set. The samples in each set were TMT-labeled as follows: Fatbody samples: WT FB MS1; 126 DP FB MS 1; 127n WT FB MS2; 127c DP FB MS 2; 128n WT FB MS3; 128c DP FB MS 3; 129n Thoracic muscles samples: Mef>Ci 1; 126 Mef>Dpi 1; 127n Mef>Ci 2; 127c Mef>Dpi 2; 128n Mef>Ci 3; 128c Mef>Dpi 3; 129n Mef>Ci 4; 129c Mef>Dpi 4; 130n

2021-02-11 | MSV000086854 | MassIVE

Single cell transcriptional profile of third instar larval wing discs in Drosophila

Project description:Our study focuses on understanding the early transcriptional changes taking place during the divergence of the adult muscle precursors that give rise to indirect flight muscles and direct flight muscles in Drosophila. We analyzed the heterogenous cell population of the adult muscle precursors by scRNA-seq and build an integrated single-cell reference atlas. We addressed the differences among muscle-type and different cell state during myoblast differentiation. Also, our dataset includes the transcriptional profile of the epithelial cells localized in the presumptive hinge and notum of third instar larval wing discs. In addition we studied the functional relevance of Amalgam in flight muscle development by depleting Ama expression specifically in the adult muscle precursors. We determined the transcriptional changes and perturbations in AMP cell identity upon Ama knockdown.

2020-08-31 | E-MTAB-9444 | biostudies-arrayexpress

First Transcriptomic insight into the working muscles of racing pigeons during a competition flight

Project description:The currently known homing pigeon is a result of a sharp one-sided selection for flight characteristics focused on speed, endurance, and spatial orientation. This has led to extremely well-adapted athletic phenotypes in racing birds. Here, we identify genes and pathways contributing to exercise adaptation in sport pigeons by applying next-generation transcriptome sequencing of m.pectoralis muscle samples, collected before and after a 300 km competition flight. The analysis of differentially expressed genes pictured the central role of pathways involved in fuel selection and muscle maintenance during flight, with a core set of genes: ARTN, NREP, CAV3, SLC25A30, SLC2A11. Variations in these genes may therefore be exploited for genetic improvement of the racing pigeon population towards specific categories of competition flights.

2023-01-15 | GSE222537 | GEO

First Transcriptomic insight into the working muscles of racing pigeons during a competition flight

Project description:First Transcriptomic insight into the working muscles of racing pigeons during a competition flight

| PRJNA922484 | ENA

The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle

2014-12-23 | GSE63707 | GEO