Transcriptomics

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Gene expression analysis of embryonic stem cells expressing VE-cadherin (CD144) during endothelial differentiation


ABSTRACT: Endothelial differentiation occurs during normal vascular development in the developing embryo. Mouse embryonic stem (ES) cells were used to further define the molecular mechanisms of endothelial differentiation. By flow cytometry a population of VEGF-R2 positive cells was identified as early as 2.5 days after differentiation of ES cells, and a subset of VEGF-R2 + cells, that were CD41+ positive at 3.5 days. A separate population of VEGF-R2+ stem cells expressing the endothelial-specific marker CD144 (VE-cadherin) was also identified at this same time point. Microarray analysis of >45,000 transcripts was performed on RNA obtained from cells expressing VEGF-R2, CD41, and CD144. Keywords: expression analysis

ORGANISM(S): Mus musculus

PROVIDER: GSE10210 | GEO | 2008/07/14

SECONDARY ACCESSION(S): PRJNA108329

REPOSITORIES: GEO

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