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Transcriptome variation among human embryonic stem cell lines impacts on their differentiation

ABSTRACT: Human embryonic stem cells (hESCs) have the pluripotency to form any cell type in the body, making them attractive cell sources in drug screening, regenerative medicine, disease and developmental processes modeling. However, not all hESC lines have equal potency to generate desired cell types in vitro, significant variation has been observed for the differentiation efficiency of various human ESC lines, and the precise underpinning molecular mechanism are still unclear. In this work, we compared transcriptome variation of four hESC lines H7, HUES1, HUES8 and HUES9 to find out whether gene expression difference between hESC lines influence their differentiation behavior in vitro. Results demonstrate that different expression genes (DEGs) are significantly enriched in developmental process, such as ectoderm, mesoderm and endoderm development, and the enrichment difference between lines is in accordance with its lineage bias. Among these DEGs, pluripotency factors and genes involved in signaling transduction also show great variation among lines. The Pleiotropic functional role of these genes in hESC identity controlling and early lineage specification, implicate that different hESC lines with distinct differentiation propensity may utilize individual balance mechanism to maintain pluripotent state, whereas the balance broken in a certain environment, gene expression variation between them could impact on their different lineage specification behavior.

ORGANISM(S): Homo sapiens

PROVIDER: GSE102311 | GEO | 2018/09/24

REPOSITORIES: GEO

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