Global fine-tuning of translation by queuosinylation and Dnmt2-dependent tRNA methylation
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ABSTRACT: In most eukaryotes, the wobble position of tRNA with a GUN anticodon is queuosine-modified (Q34). Q is synthesized exclusively by eubacteria and salvaged by eukaryotes as a nutrient to replace G34 in tRNAs. Q34 modification stimulates Dnmt2/Pmt1-dependent C38 methylation in the tRNAAsp anticodon loop in Schizosaccharomyces pombe. Due to the location of both modification in the anticodon loop, we anticipated an influence on translation. Our experimental setup allowed for the analysis of effects from each modification alone or a combination of both.
ORGANISM(S): Schizosaccharomyces pombe
PROVIDER: GSE102376 | GEO | 2019/02/13
REPOSITORIES: GEO
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