C-Maf-dependent transcripts in effector Tregs from Peyer's patches
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE102491 | GEO | 2017/11/11
SECONDARY ACCESSION(S): PRJNA397859
REPOSITORIES: GEO
Similar Datasets
Project description:Homozygous disruption of c-Maf led to embryonic lethality and impaired erythroblastic island formation. c-Maf is expressed in the fetal liver macrophages. It suggests that macrophages are responsible for the lethality of c-Maf knock-out embryos. To search downstream genes of c-Maf, we surveyed genes associated with macrophage function by microarray analysis. keywords: c-Maf, macrophage, erythroblastic islands, WT (c-Maf WT) and c-Maf KO (c-Maf KO) fetal liver macrophages were sorted by a FACSAria cell sorter. Total RNAs from those macrophages were prepared using RNeasy Kit. Genes down-regulated in c-Maf KO macrophages were searched by GeneSpring software.
2011-07-06 | E-GEOD-23305 | biostudies-arrayexpress
Project description:Retinoid-related orphan receptors RORalpha and RORgamma are both expressed in liver; however, their physiological functions have not yet been clearly defined. RORalpha1 and RORgamma1, but not RORalpha4, show an oscillatory pattern of expression during circadian rhythm. To obtain insight into the physiological functions of ROR receptors in liver, we analyzed the gene expression profiles of livers from WT, RORalpha-deficient staggerer mice (RORalphasg/sg), RORgamma-/-, and RORalpha sg/sgRORgamma-/- double knockout (DKO) mice by microarray analysis. DKO mice were generated to study functional redundancy between RORalpha and RORgamma. These analyses demonstrated that RORalpha and RORgamma affect the expression of a number of genes. RORalpha and RORalpha are particularly important in the regulation of genes encoding several Phase I and Phase II metabolic enzymes, including several 3beta-hydroxysteroid dehydrogenases (Hsd3b), the cytochrome P450 (Cyp) enzymes Cyp7b1, Cyp4a14, and Cyp8b1, and the sulfotransferases Sult1e1 and Sult2a1. Our data show that RORalpha and RORgamma can influence gene expression positively as well as negatively. In addition, our results indicate that RORalpha and RORgamma each affect the expression of a specific set of genes but also exhibit functional redundancy. Our study shows that RORalpha and RORgamma receptors influence the regulation of several metabolic pathways, including those involved in the metabolism of steroids, bile acids, and xenobiotics, suggesting that RORs are important in the control of metabolic homeostasis. Experiment Overall Design: We generated RORalpha and RORgamma double knockout (DKO) mice and compared the gene expression profiles of livers from wild type (WT), RORalphasg/sg, RORgamma-/-, and DKO mice by microarray analysis
2008-06-15 | E-GEOD-7564 | biostudies-arrayexpress
Project description:Foxp3-expressing regulatory T (Treg) cells are critical mediators of immunological tolerance to both self and microbial antigens. Tregs activate context-dependent transcriptional programs to adapt effector function to specific tissues; however, the factors controlling tissue-specific gene expression in Tregs remain unclear. Here, we find that the AP-1 transcription factor JunB regulates the intestinal adaptation of Tregs by controlling select gene expression programs in multiple Treg subsets. Treg-specific ablation of JunB results in immune dysregulation characterized by enhanced colonic T helper cell accumulation and cytokine production. However, in contrast to its classical binding-partner BATF, JunB is dispensable for maintenance of effector Tregs as well as most specialized Treg subsets. In the Peyer’s patches, JunB activates a transcriptional program facilitating the maintenance of CD25- Tregs, leading to the complete loss of T follicular regulatory cells in the absence of JunB. This defect is compounded by loss of a separate effector program found in both major colonic Treg subsets that includes the cytolytic effector molecule granzyme B. Therefore, JunB is an essential regulator of intestinal Treg effector function through pleiotropic effects on gene expression.
2020-02-26 | GSE145888 | GEO
Project description:We observed significant growth inhibition of melanoma tumor in ROR?-/- mice, suggesting an important role for the T helper 17 cell (TH17) pathway in tumor immunity. Though RORgamma t (ROR?t) plays a critical role in the development of IL-17-secreting Th17 cells, the role of other genes remained an open question. To explore the expression of unknown genes with anti-tumor properties, gene expression analysis was performed. Sorted naïve TH cells (CD4+CD25-CD62Lhigh) from RORc+/+ and RORc-/-mice were differentiated under TH17 polarizing conditions. After 4 days, cells were harvested, RNA was isolated and gene expression analysis was performed.
2013-04-17 | E-GEOD-38704 | biostudies-arrayexpress
Project description:Transcription factor Maf-S knockdown by dsRNA injection and compared to dsRNA-GFP injected control. Maf-S knockdown was performed to study the affect of xenobiotic stress of the cnc-keap1 pathway.
2017-03-19 | E-MTAB-4042 | biostudies-arrayexpress
Project description:Retinoid-related orphan receptors RORalpha and RORgamma are both expressed in liver; however, their physiological functions have not yet been clearly defined. RORalpha1 and RORgamma1, but not RORalpha4, show an oscillatory pattern of expression during circadian rhythm. To obtain insight into the physiological functions of ROR receptors in liver, we analyzed the gene expression profiles of livers from WT, RORalpha-deficient staggerer mice (RORalphasg/sg), RORgamma-/-, and RORalpha sg/sgRORgamma-/- double knockout (DKO) mice by microarray analysis. DKO mice were generated to study functional redundancy between RORalpha and RORgamma. These analyses demonstrated that RORalpha and RORgamma affect the expression of a number of genes. RORalpha and RORalpha are particularly important in the regulation of genes encoding several Phase I and Phase II metabolic enzymes, including several 3beta-hydroxysteroid dehydrogenases (Hsd3b), the cytochrome P450 (Cyp) enzymes Cyp7b1, Cyp4a14, and Cyp8b1, and the sulfotransferases Sult1e1 and Sult2a1. Our data show that RORalpha and RORgamma can influence gene expression positively as well as negatively. In addition, our results indicate that RORalpha and RORgamma each affect the expression of a specific set of genes but also exhibit functional redundancy. Our study shows that RORalpha and RORgamma receptors influence the regulation of several metabolic pathways, including those involved in the metabolism of steroids, bile acids, and xenobiotics, suggesting that RORs are important in the control of metabolic homeostasis. Keywords: knockout comparison
2007-08-24 | GSE7564 | GEO
Project description:Transcriptome analyses were done comparing gene regulation of TFH and TFR cells isolated from Peyer’s Patches (PP) and immunized peripheral lymph nodes (pLN) revealing many regulatory patterns common to all follicular cells. However, in contrast to TFH cells, the kinetic but also the extent of regulation regarding many genes was attenuated in pLN TFR cells when compared to those of PP. Additionally, PP but not pLN TFR cells were largely unresponsive to IL2 and expressed Il4 as well as Il21. Together with fundamental differences in gene expression that were found between cells of both compartments this emphasizes specific adaptations of follicular T cell functions to their micro-milieu.
2018-04-16 | GSE106590 | GEO
Project description:Transcriptome analyses were done comparing gene regulation of TFH and TFR cells isolated from Peyer’s Patches (PP) and immunized peripheral lymph nodes (pLN) revealing many regulatory patterns common to all follicular cells. However, in contrast to TFH cells, the kinetic but also the extent of regulation regarding many genes was attenuated in pLN TFR cells when compared to those of PP. Additionally, PP but not pLN TFR cells were largely unresponsive to IL2 and expressed Il4 as well as Il21. Together with fundamental differences in gene expression that were found between cells of both compartments this emphasizes specific adaptations of follicular T cell functions to their micro-milieu.
2018-04-16 | GSE106490 | GEO
Project description:RAR-related orphan receptor gamma (ROR t)-expressing regulatory T (ROR t+ Treg) cells play pivotal roles in preventing T cell hyperactivation and maintaining tissue homeostasis, in part, by secreting the anti-inflammation cytokine interleukin 10 (IL-10). Here, we report that Hypoxia Induced Factor 1a (HIF1alpha) is the master transcription factor for *Il10* in ROR t+ Tregs. Interestingly, this critical anti-inflammatory pathway is negatively regulated by an RNA-binding protein DEAD box helicase 5 (DDX5). As a transcriptional corepressor, DDX5 restricts the expression of HIF1 and its downstream target gene *Il10* in ROR t+ Tregs. T cell specific *Ddx5* knockout (DDX5 T) mice have augmented ROR t+ Treg suppressor activities and are better protected from intestinal inflammation. Genetic ablation or pharmacologic inhibition of HIF1a restores enteropathy susceptibility in DDX5 T mice. The DDX5-HIF1 -IL-10 pathway is conserved in mice and humans. These findings reveal potential therapeutic targets for intestinal inflammatory diseases.
2022-12-21 | GSE157259 | GEO
Project description:We performed genome-wide mapping of MAF binding sites in control and MAF-overexpressing MCF7 cells to assess the consequences of estrogen (E2) stimulation on MAF recruitment to chromatin. To this end, we cultured MCF7 cells in hormone-deprived (HD) medium for 72 h and then E2 or vehicle was added for 1h prior to chromatin immunoprecipitation (ChIP). Samples were generated in triplicate. We report that MAF binding is largely independent of E2
2023-09-15 | GSE210605 | GEO