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Highly multiplexed and quantitative cell surface protein profiling using genetically barcoded antibodies

ABSTRACT: Next generation sequencing (NGS) allows for sensitive quantification of DNA and RNA. It would be highly desirable to have a systematic equivalent for assaying cellular protein levels on living cells. We present a highly multiplexed, quantitative, and inexpensive sequencing-based proteomic method using genetically barcoded antibodies called Phage-antibody Next Generation Sequencing (PhaNGS). We demonstrate the utility of PhaNGS by showing how a set of 144 targeted Fab-phage can reliably detect changes in 44 targeted cell surface proteins in drug sensitive and resistant B-cells, or upon induction of the Myc oncogene.

ORGANISM(S): Homo sapiens

PROVIDER: GSE102712 | GEO | 2018/02/12

REPOSITORIES: GEO

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