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The Arabidopsis thaliana wild-type (Ws-2) and CLAVATA1 (clv1.12 and clv1.13) mutant transcriptomes upon the compatible interaction with Hyaloperonospora arabidopsidis

ABSTRACT: We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations in wild-type plants and clv mutants, 3 days after water treatment and inoculation with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE102754 | GEO | 2017/08/18

SECONDARY ACCESSION(S): PRJNA398616

REPOSITORIES: GEO

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Arabidopsis thaliana/Hyaloperonospora arabidopsidis compatible interaction transcriptome

Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations at different stages of infection with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis : initiation (< 1 dpi) and maintenance of infection (> 4 dpi). In two independent experiments, cotyledons from the ecotype Wassilewskija (WS) were inoculated with water, or with Hyaloperonospora arabidopsidis to establish a compatible interaction. Affymetrix ATH1 microarrays were used to profile Arabidopsis transcript accumulations at the initiation (mixed samples at 8 and 24 hours post inoculation, hpi; early stage) and maintenance (mixed samples at 4 and 6 days post inoculation; late stage) of the compatible interaction.

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Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations in wild-type plants and pskr1-5 mutants, 3 days after water treatment and inoculation with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis. In two independent experiments, cotyledons from the wild-type Wassilewskija (WS) ecotype and from the pskr1-5 mutant were treated with water, or inoculated with the H. arabidopsidis isolate Emwa1 to establish a compatible interaction. Affymetrix ATH1 microarrays were used to profile Arabidopsis transcript accumulations at 3 days after onset of treatment.

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Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations in map65-3 and ugt76b1 mutants, 3 days after water treatment and inoculation with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis (Hpa) In two independent experiments, cotyledons from the wild-type Wassilewskija (WS) ecotype, and from the map65-3 and ugt76b1 mutants were treated with water, or inoculated with the Hpa isolate Emwa1 to establish a compatible interaction. Affymetrix ATH1 microarrays were used to profile Arabidopsis transcript accumulations at 3 days after onset of treatment. Data from the water-treated and Hpa-infected wild-type were previously deposited as GSM914964, GSM914965, GSM914966, and GSM914967. The wild-type data, and the data from the map65-3 and ugt76b1 mutant presented here were established in the same set of experiments and analyses, which also involved the previously deposited pskr1-5 mutant (GSM914968, GSM914969, GSM914970, and GSM914971).

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