Project description:Transcriptional profiling of chemoautotrophically and arabinose supplemented chemoautotrophically grown cells Keywords: Comparison of different lifestyles
Project description:Transcriptional profiling of arabinose supplemented chemoautotrophically grown cells Keywords: Comparison of different lifestyles Three independent biological materials were prepared for arabinose supplemented chemoautotrophically cultured cells and heterotrophically cultured cells. Total 6 arrays including dye swap were analyzed.
Project description:Transcriptional profiling of chemoautotrophically and arabinose supplemented chemoautotrophically grown cells Keywords: Comparison of different lifestyles Three independent biological materials were prepared for arabinose supplemented chemoautotrophically cultured cells and chemoautotrophically cultured cells. Total 6 arrays including dye swap were analyzed.
Project description:Transcriptional profiling of chemoautotrophically grown cells Keywords: Comparison of different lifestyles Two independent biological materials were prepared for chemoautotrophically cultured cells and heterotrophically cultured cells. Total 4 arrays including dye swap were analyzed.
Project description:Role of L-arabinose on gene expression in E. coli O157:H7 TUV93-0 where CTRL samples were grown in the absence of L-arabinose and ARA samples were grown in the presence of L-arabinose.
Project description:Transcriptional profiling of A. niger comparing different strains (WT, ΔXlnR, ΔaraR and ΔaraRΔXlnR) treated with 25mM xylose + 25mM arabinose for 2 and 8h. The main objective was to identifiy genes related to primary metabolism and carbohydrate metabolism in general, for instance, cellulases and hemicellulases. The study will compare the differences between WT strain and the strains with the disrupted xylanolytic transcriptional activator gene, XlnR, the arabinolytic transcriptional activator gene, AraR, and the double mutant,after treatment with xylose+arabinose (XA treatment). The experiment was further validated by real-time PCR and enzymatic assays.
Project description:Transcriptional profiling of A. niger comparing different strains (WT, M-NM-^TXlnR, M-NM-^TaraR and M-NM-^TaraRM-NM-^TXlnR) treated with 25mM xylose + 25mM arabinose for 2 and 8h. The main objective was to identifiy genes related to primary metabolism and carbohydrate metabolism in general, for instance, cellulases and hemicellulases. The study will compare the differences between WT strain and the strains with the disrupted xylanolytic transcriptional activator gene, XlnR, the arabinolytic transcriptional activator gene, AraR, and the double mutant,after treatment with xylose+arabinose (XA treatment). The experiment was further validated by real-time PCR and enzymatic assays. Two-condition experiment : A. niger mutant strains on XA for 2 and 8 h at 30 oC in batch culture. Firstly, the strains were pre-grown in minimal medium with fructose as carbon source (control), and then transferred to xylose+arabinose (25 mM each) as carbon source.
Project description:This SuperSeries is composed of the following subset Series: GSE10295: Bj_Heterotrophy vs. Arabinose supplemented chemoautotrophy GSE10296: Bj_Heterotrophy vs. Chemoautotrophy GSE10298: Bj_Chemoautotrophy vs. Arabinose supplemented chemoautotrophy Refer to individual Series
Project description:D-glucose, D-xylose and L-arabinose are three major monosaccharides in the plant cell wall. Complete utilization of all three sugars is still a bottleneck for second-generation cellulolytic bioethanol production, especially for L-arabinose. However, little is known about the gene expression profiles during L-arabinose utilization in fungi at genome-wide level and a comparison of the genome-wide fungal response to these three major monosaccharides has not yet been reported. To better understand the microbial response and utilization of L-arabinose for subsequent microbial engineering for co-fermentation all three sugars, we performed transcriptome analysis of N. crassa grown on L-arabinose, compared with that on D-xylose and D-glucose.