Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq (Drop-seq) of MGE, CGE and LGE of E13.5 (MGE) and E14.5 (CGE, LGE) mouse embryos

ABSTRACT: Single-cell RNA-seq to profile a developmental time course of interneuron development, measuring the transcriptomes of progenitors during their maturation in the ganglionic eminences.

ORGANISM(S): Mus musculus

PROVIDER: GSE103983 | GEO | 2018/03/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell RNA-seq (10X Genomics Chromium) of MGE, CGE, cortical and subcortical regions of E13.5, E18.5 and P10 mice

Project description:Single-cell RNA-seq to profile a developmental time course of interneuron development, measuring the transcriptomes of progenitors during their maturation in the ganglionic eminences.

2018-03-05 | GSE104156 | GEO

Single-cell RNA-seq (10X Genomics Chromium) of LGE, MGE, CGE, and Cortex from mouse embryonic forebrain

Project description:We performed single-cell RNA sequencing to characterize transcriptional heterogeneity of neural progenitors within the ventricular zone and subventricular zone of E12.5 and E14.5 mouse cortex and ganglionic eminences. By using a transgenic mouse line (Nestin-d4-Venus) to enrich for VZ cells, we detect significant transcriptional heterogeneity within VZ and SVZ progenitors, both between forebrain regions and within spatial subdomains of specific GEs.

2022-02-28 | GSE190593 | GEO

Developmental diversification of cortical inhibitory interneurons

Project description:We applied massively parallel single-cell RNA-seq to profile a developmental time course of interneuron development, measuring the transcriptomes of over 60,000 progenitors during their maturation in the ganglionic eminences and embryonic migration into the cortex.

2018-03-05 | GSE104158 | GEO

Single-cell RNA-seq (Drop-seq) of MGE, CGE and LGE of E13.5 (MGE) and E14.5 (CGE, LGE) mouse embryos

Project description:Single-cell RNA-seq (Drop-seq) of MGE, CGE and LGE of E13.5 (MGE) and E14.5 (CGE, LGE) mouse embryos

| PRJNA407881 | ENA

MGE, CGE, LGE and cortical regions from mouse embryonic forebrain

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-01 | GSE201494 | GEO

Arx acts as a key selector gene of the ventral telencephalon mainly through its repression transcriptional activity

Project description:The homeobox containing gene Arx is expressed during ventral telencephalon development and it is required for correct GABAergic interneuron tangential migration from the ganglionic eminences to the olfactory bulbs, cerebral cortex and striatum. Its human ortholog is associated with a variety of neurological clinical manifestations whose syntoms are compatible with a loss of cortical interneurons and altered basal ganglia related-activities in humans. Herein, we reported the identification by global expression profiling of a group of genes whose expression is consistently altered in Arx mutant ganglionic eminences. Following analysis revealed the striking ectopic expression in the ganglionic eminences of a number of genes normally not, or only marginally, expressed in the ventral telencephalon. Among them, we functionally analyzed Ebf3, whose ectopic expression in ventral telencephalon is preventingneuronal tangential migration. Further, we showed that Arx is sufficient to repress Ebf3 endogenous expression and that its silencing in Arx mutant tissue might marginally rescue tangential cell movements. Together, these data provide an initial analysis of the molecular pathways regulated by Arx and how their networking might regulate those specific cellular processes during telencephalon development strongly altered by loss of Arx. Three timed-pregnant Arx heterozygous dams crossed with C57Bl/6 males were sacrificed at E14.5, the embryos harvested and placed into cold PBS. After brain isolarion, meninges and olfactory bulbs were removed, and the ventral telencephalon separated from the overlying cerebral cortex. The same procedure was repeated for 5 wt and 5 Arx mutant embryos.

2010-03-09 | E-GEOD-12956 | biostudies-arrayexpress

scRNA-seq of e14.5 MGE - WT vs. St18(-/-)

Project description:Medial ganglionic eminence was dissected from e14.5 mouse embryonic brain, dissociated and processed using 10X Genomics chemistry for single cell library creation. 2 experimental groups were analyzed: St18 null and wildtype littermate controls (2 biological replicates of each group)

2022-12-08 | GSE181349 | GEO

Arx acts as a key selector gene of the ventral telencephalon mainly through its repression transcriptional activity

2010-02-26 | GSE12956 | GEO

Single-cell RNA-seq (Smart-seq2) of MGE of E13.5 mice

Project description:Single-cell RNA-seq of mitotically enriched cells in the medial ganglionic eminence (Dlx6 negative), as well as labeled cells using FlashTag technology.

2018-03-05 | GSE104157 | GEO

Hi-C in MGE, CGE, LGE and cortical regions from mouse embryonic forebrain

Project description:While comparative genetic analysis has been performed between distinct regions of the embryonic forebrain, a comprehensive characterization of differential epigenomic organization has not been performed. We collected single-cell chromatin accessibility profiles from four distinct neurogenic regions of the embryonic mouse forebrain using single nuclei ATAC-Seq (snATAC-Seq). We identified thousands of differentially accessible peaks, many restricted to distinct progenitor cell types or brain regions. We integrated snATAC-Seq and single cell transcriptome data to characterize changes of chromatin accessibility at enhancers and promoters that were tightly coupled to transcript abundance. Multi-modal integration of histone modifications (CUT&Tag and CUT&RUN), promoter-enhancer interactions (Capture-C) and high-order chromatin structure (Hi-C) extended these initial observations. This dataset reveals a diverse chromatin landscape with region-specific regulatory mechanisms and genomic interactions in distinct neurogenic regions of the embryonic mouse brain and represents an extensive public resource of a ‘ground truth’ epigenomic landscape at this critical stage of neurogenesis.

2022-07-01 | GSE201186 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data