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ChIP-Seq in mouse embryonic stem cells of endogenous HA-tagged Tet1

ABSTRACT: R-loops are prevalent three-stranded nucleic acid structures, composed of a DNA:RNA hybrid and a displaced single-stranded DNA loop. Although aberrant R-loop formation is a threat to genome stability, R-loops are enriched at CpG islands (CGI) and are implicated in maintaining CGI promoters unmethylated. Mapping of genomic regions where Tet1 binds in an R-loop dependent manner we here identify almost exclusively CGIs. The results suggest that R-loops are genomic address labels for TET1.

ORGANISM(S): Mus musculus

PROVIDER: GSE104067 | GEO | 2018/10/16

REPOSITORIES: GEO

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TET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [DREAM]

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