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RNA transcription profile of different yeast mutants under glucose starvation (0.05% glucose) and comparison of transcriptome of WT and H3D_H3H

ABSTRACT: Modifications on histone tails largely affect chromatin associated processes. Previous studies have shown the existence of asymmetrically modified nucleosomes in promoters in multiple cell types. However, whether modifications on both sister histones contribute equally to chromatin dynamics remains elusive. Here we devised a bivalent nucleosome system which allows for the constitutive assembly of asymmetrically modified sister histone H3 in nucleosomes in vivo. We found that the H3K36 methylation on either of sister histones sensitize chromatin structure in gene coding region, while the H3K79 methylation on both sister histone H3s are indispensable for maintaining silent chromatin near telomeres. Additionally, the H3K4 methylation on sister histones plays an equivalent role in suppressing the recruitment of Gal4 activator at GAL1 promoter and GAL1 transcription. Moreover, under starvation stress the expression of a significant proportion of genes in the whole genome is affected by the lack of the H3K4 methylation on one tail of the sister histones, indicating that both sister histones function in fine-tuning transcription through their modifications. We conclude that modifications on sister histones either cooperatively or independently respond to the change of an environmental cue to regulate chromatin structure and function. This work provides a unique experimental strategy to investigate the cross talk between sister histones, and for the first time elaborate their contributions to chromatin in vivo.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE104312 | GEO | 2017/10/16

SECONDARY ACCESSION(S): PRJNA412318

REPOSITORIES: GEO

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RNA transcription profile of different yeast mutants under glucose starvation (0.05% glucose)

Project description:Histone tail modificationscan greatly influencechromatin-associated processes. Asymmetrically modified nucleosomes exist inmultiple cell types; however,whether modifications on both sister histones contribute equally to chromatin dynamicsremainselusive. Here, we devised a bivalent nucleosome system thatallowedfor the constitutive assembly of asymmetrically modified sister histone H3s in nucleosomes inSaccharomyces cerevisiae. The sister H3K36 methylationsaffectedcryptic transcription in gene coding regions, whereassister H3K79 methylation had cooperative effectsongene silencing near telomeres.H3K4 methylation on sister histonesplayed an independent role in suppressing the recruitmentof the Gal4 activator and inhibitingGAL1transcription. Under starvation stress, the expression of glycometabolism pathway geneswasfinetuned by sisterH3K4 methylation.Thus,different modifications on sister histones act either cooperatively or independentlyto regulate transcription in response to external stimuli.

2017-10-16 | GSE88878 | GEO

MARCS: Modification Atlas for Regulation by Chromatin States

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Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation

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2017-01-15 | GSE93059 | GEO

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