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DREAM-seq, an endonucleases-assisted mapping of R-loops

ABSTRACT: Genome-wide identification of R-loops almost uniquely relies on antibody based pull-down, a method that shows sequence and structural biases. Here we describe a novel approach – DREAM-seq – centered around the use of endonuclease digestions. Unstimulated and stimulated B cells showed that R-loops are more widespread than anticipated, mostly covering all actively transcribed genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE104413 | GEO | 2020/09/26

REPOSITORIES: GEO

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