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Affymetrix Gene Expression profiles of murine LAMTOR2-deficient hematopoietic progenitor cells

ABSTRACT: LAMTOR2, a member of the Ragulator complex, controls endosomal signaling. Here, we show that postnatal Mx1-Cre-driven deletion of LAMTOR2 in mice resulted in the development of a cell-intrinsic myeloproliferation characterized by splenomegaly, accumulation of myelomonocytoid cells, and depletion of long-term hematopoietic stem cells. LAMTOR2-deficient hematopoietic progenitors exhibited a myeloid-skewed gene expression signature as well as an enhanced GM-CSF-mediated colony formation and activation of ERK signaling. In contrast, disruption of LAMTOR2 resulted in decreased G CSF-mediated STAT3 signaling and impaired granulocytic differentiation. LAMTOR2-defective cells showed aberrant multivesicular body formation, consistent with delayed degradation of activated cytokine receptors. Our study reveals a critical role of LAMTOR2 in regulating cytokine signaling during hematopoietic differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE104444 | GEO | 2019/12/31

REPOSITORIES: GEO

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