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ChIP-seq Analysis of MCF7 Cells on Treatment with Estradiol with internal controls

ABSTRACT: A key challenge in quantitative ChIP-seq is the normalisation of data in the presence of genome-wide changes. Data-based methods often rely on assumptions that do not hold true. Misapplication of these methods to ChIP-seq data results in the suppression of the biological signal or erroneous measurement of differential occupancy. To develop methods that address this challenge, we generated two ChIP-seq datasets, measuring Estrogen Receptor-alpha (ER) binding and H4K12ac histone marks in MCF7 before and after 100 nm Estradiol treatment for 45 minutes and 2 hours respectively.

ORGANISM(S): Homo sapiens

PROVIDER: GSE107749 | GEO | 2017/12/07

SECONDARY ACCESSION(S): PRJNA421277

REPOSITORIES: GEO

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ChIP-seq Analysis of Estrogen Receptor-alpha on Treatment with Fulvestrant with internal and spike-in controls

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