Project description:Analysis of the contribution of the post-transcriptional regulator CsrA to translation during exponential growth in Escherichia coli
Project description:Analysis of the contribution of the post-transcriptional regulator CsrA to translation during exponential growth in Escherichia coli
Project description:Analysis of the contribution of the post-transcriptional regulator CsrA to RNA stability during exponential growth in Escherichia coli
Project description:Analysis of the contribution of the post-transcriptional regulator CsrA to RNA stability in Salmonella during growth in LB and mLPM media
Project description:This RNA-seq analysis examined the global effect of CsrA and its non-coding small RNA (ncsRNA) csrB in E. amylovora under the T3SS-inducing condition. The csrA mutant showed differential expression of more than 20% genes in the genome, which include significant down-regulation of T3SS genes and those required for cell growth and viability. On the other hand, the csrB mutant exhibited significant up-regulation of most major virulence genes, suggesting antagonistic effect of csrB on CsrA targets.
Project description:The RNA content of the CsrA foci. EPEC (E2348/69 (strain O127:H6)) expressing CsrA-FLAGx3-GFP or wild-type EPEC expressing untagged CsrA (negative control) were grown to OD 0.6 in DMEM and subjected to the foci-enrichment protocol, followed by RNA extraction and library preparation. Six libraries were prepared, containing three biological repeats of csrA-3 x flag-gfp and three repeats of wt.
Project description:Analysis of RNA binding partners of the post-transcriptional regulator CsrA to translation during exponential growth in Escherichia coli
Project description:Analysis of the contribution of the post-transcriptional regulator CsrA to translation during exponential growth in Escherichia coli
Project description:Purpose: The goals of this study are to clarify the CsrA-regulatory system in H. pylori by NGS-derived transcriptome profiling (RNA-seq). Method: CsrA regulatory system was determined by comparative transcriptomic analysis carried out with RNA-seq on strain J99 and csrA mutant. Results: Using an optimized data analysis workflow, fifty-three genes in the csrA mutant were found to be differentially expressed compared with the wild-type. Conclusions: Our study represents the first detailed analysis of CsrA-regulatory system in H. pylori J99.
