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Directed evolution of reprogramming factors by cell selection and sequencing

ABSTRACT: Directed biomolecular evolution is widely used to tailor and enhance proteins but has hitherto not been applied in the reprogramming of mammalian cells. Here we describe a novel method to identify artificially enhanced and evolved reprogramming factors by pooled screens with randomised protein libraries, cell selection based on phenotypic readouts and genotyping by amplicon sequencing. We benchmark this approach by identifying artificially enhanced Sox2 and Sox17 factors in pluripotency reprogramming.

ORGANISM(S): Mus musculus

PROVIDER: GSE107987 | GEO | 2018/07/12

REPOSITORIES: GEO

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