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Transcriptomics study of three Blumeria graminis formae speciales

ABSTRACT: We analyzed the gene expression and especially the expression of effector candidate genes in wheat and rye powdery mildews.

ORGANISM(S): Triticum aestivum Blumeria graminis f. sp. secalis Secale cereale Blumeria graminis f. sp. tritici Blumeria graminis f. sp. triticale x Triticosecale

PROVIDER: GSE108405 | GEO | 2018/01/10

SECONDARY ACCESSION(S): PRJNA427159

REPOSITORIES: GEO

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

Project description:Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChipM-BM-. Rice Genome Array. To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChipM-BM-. Rice Genome Array hybridization. For transcript proM-oM-,M-^Aling experiments with powdery mildew, entire leaves were sampled from rice plantlets 2 weeks old (cv. Nipponbare). Treated and control (mock) rice leaves were collected 24 hours post inoculation. Three biological replicates for inoculated and control plants were extracted and analysed independently with the GeneChipM-BM-. Rice Genome Array.

2012-03-26 | E-GEOD-30942 | biostudies-arrayexpress

Transcriptomics study of three Blumeria graminis formae speciales

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Transcriptomic analysis of Blumeria graminis triticale on different hosts

Project description:With this experiment we aimed do identify eventual genes that are differentially expressed by the fungal pathogen Blumeria graminis triticale when it grows on two different hosts (wheat and triticale) We used to fungal isolates, for each of them we infected wheat and triticale and we extracted RNA (and sequenced) from the infected plant tissue. Three technical replicates for each combinations plant-pathogen were used

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