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Transcriptome of Dmrt3 KO, Dmrt5 KO, Dmrt3;Dmrt5 double KO and wildtype cortical tissue at E12.5.

ABSTRACT: Here we generated transcriptomes of dissected cortical tissue of Dmrt3 and Dmrt5 single knockout, of Dmrt3;Dmrt5 double knock-out and wildtype littermate at embryonic day 12.5.

ORGANISM(S): Mus musculus

PROVIDER: GSE108611 | GEO | 2018/12/28

REPOSITORIES: GEO

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Transcriptome of Dmrt3 KO, Dmrt5 KO, Dmrt3 Dmrt5 double KO and wildtype cortical tissue at E12.5.

Project description:Transcriptome of Dmrt3 KO, Dmrt5 KO, Dmrt3 Dmrt5 double KO and wildtype cortical tissue at E12.5.

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Prenatal exposure to valproic acid induces sex specific alterations in cortical and hippocampal neuronal structure and function in rats

Project description:In this dataset we include the expression data obtained by dissected prefrontal cortical tissue from sprague-dawley rats exposed to saline or valproic acid on gestation day 12.5 in utero. This data was used to obtain prefrontal cortical genes to determine sex differences in a model of autism spectrum disorder.

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Gene expression affected by loss-of- or gain-of-function of Dmrt3/Dmrta2 in the dorsomedial telencephalon

Project description:We then examined the mutant mice that lack both Dmrt3 and Dmrta2 alleles, and found the size of the medial cortex including the hippocampus and the cortical hem was significantly reduced in mutant brains .To further investigate how Dmrt3 and Dmrta2 are involved in the development of the medial cortex, we applied gene expression profiling approach using whole genome DNA microarray to identify genes which are differentially expressed in Dmrt3/Dmrta2-knockdown cells. Cortical cells knocked-down both Dmrt3 and Dmrta2 exhibited a dramatic increase of Pax6 expression in the medial cortex, leading to the precocious neuronal differentiation, and simultaneous knockdown of Pax6 with Dmrt3/Dmrta2 rescued the phenotype. Our results unveil a key mechanism that mammalian Dmrt factors maintain more proliferative potential of neural progenitors by suppressing Pax6 expression in the developing cerebral cortex.

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E12.5 Murine lateral cortical progenitor cells: Pax6sey/sey vs. Pax6+/+

Project description:The mechanisms by which the early spatiotemporal expression patterns of transcription factors such as Pax6 regulate the region-specific proliferation rates of neural progenitors are poorly understood. Pax6 is expressed in a gradient across the developing cortex and is essential for normal corticogenesis. We found that constitutive or conditional loss of Pax6 increases cortical progenitor proliferation by amounts that vary regionally with normal Pax6 levels. We compared the gene expression profiles of equivalent Pax6-expressing progenitors isolated from Pax6+/+ and Pax6-/- cortices and identified many negatively-regulated cell cycle genes including those encoding Cyclins and Cdks. Biochemical assays indicated that Pax6 directly represses Cdk6 expression. Cyclin/Cdk repression inhibits retinoblastoma protein (pRb) phosphorylation, thereby limiting the transcription of genes directly promoting mitosis, and we showed that Pax6 inhibits pRb phosphorylation and represses several genes involved in DNA replication. Our results indicate that Pax6M-bM-^@M-^Ys modulation of the cell cycles of cortical progenitors is regional and direct. Two condition experiment, Pax6sey/sey vs Pax6+/+ cortical progenitor cells from lateral cortex. 3 biological replicates. Each replicate contains RNA from cells pooled from 4 individual E12.5 embryos

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Transcription factors Bcl11a and Bcl11b are Required for the production and differentiation of cortical projection neurons

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SLMAP3 protein interactions in e12.5 mouse brains

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Regulating lineage progression of cortical neural stem cells by extrinsic signaling molecule SHH [single-cell RNA-seq]

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E12.5 Murine lateral cortical progenitor cells: Pax6sey/sey vs. Pax6+/+

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BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic period

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