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On the design of CRISPR-based single cell molecular screens

ABSTRACT: We demonstrate that vector designs for such screens that rely on cis linkage of guides and distally located barcodes suffer from swapping of intended guide-barcode associations at rates approaching 50% due to template switching during lentivirus production, greatly reducing sensitivity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE108699 | GEO | 2018/01/29

REPOSITORIES: GEO

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