Small-molecule-based Human Genome G4 Profiling Reveals Potential Gene Regulation Activity
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ABSTRACT: G-quadruplexes (G4s) are a type of secondary structure of nucleic acids, the formation of which depends on the π-π stacking of guanine-rich sequences. Herein, we present a readily accessible strategy, based on small molecule pulldown, for direct genomic G4 profiling of HEK 293T cells. A small molecule was rationally designed, containing PDP on one end and biotin on the other, joined by a 24-unit-long hydrophilic PEG linker. G4s isolation from genomic DNA annealed in potassium chloride buffer beforehand, has identified a total of 51446 PQS, which mainly distribute in introns and intergenic regions. Incubating cells with PDP for 3 h to 72 h resulted in the differential expression of a variety of genes, indicating a potential function of G4s in gene regulation.
ORGANISM(S): Homo sapiens
PROVIDER: GSE109369 | GEO | 2019/02/15
REPOSITORIES: GEO
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