Integrative analysis of methylomic and transcriptomic data in fetal sheep muscle tissues in response to maternal diet during pregnancy
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ABSTRACT: Numerous studies have established a link between maternal diet and the physiological and metabolic phenotypes of the offspring. In previous studies in sheep, we demonstrated that different maternal diets had altered the transcriptome of fetal tissues. However, the mechanisms underlying those transcriptomic changes are poorly understood. DNA methylation is an epigenetic mark regulating transcription and is largely influenced by dietary components of the one-carbon cycle, a generator of the methyl group donor. Therefore, in the present study, we hypothesized that different maternal diets during pregnancy will alter the DNA methylation and gene expression patterns in fetal tissues. Pregnant ewes were randomly divided into two groups each of which received a hay or corn diet from mid-gestation (day 67±5) until necropsy (day 131 ± 1). The diets were formulated to meet the nutritional needs of ewes during pregnancy. On the day of necropsy, longissimus dorsi (LD) muscle tissues were collected from the fetuses and then used for DNA methylation analysis and gene expression profiling.
ORGANISM(S): Ovis aries
PROVIDER: GSE109512 | GEO | 2018/01/24
REPOSITORIES: GEO
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